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Media Required for Plant Cell Culture
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Question
Which of the following is an ethylene biosynthesis inhibitor?
Options
A. Citric acid
B. Succinic acid
C. Activated charcoal
D. Silver thiosulphate
Correct Answer
Silver thiosulphate
Media Required for Plant Cell Culture problems
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1. Which is the most common carbon source used in the plant cell culture media?
Options
A. Sucrose
B. Glucose
C. Fructose
D. Maltose
Show Answer
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Discuss
Correct Answer: Sucrose
2. Which of the following is not an auxin?
Options
A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. zeatin
D. Indole butyric acid
Show Answer
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Correct Answer: zeatin
3. Which of the following growth regulator promote cell division?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Discuss
Correct Answer: Cytokinins
4. Silver thiosulphate is added to culture medium as it helps to
Options
A. maintain the pH
B. remove toxic phenolics from plant cells
C. prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
D. all of the above
Show Answer
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Discuss
Correct Answer: prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
5. What is 'nurse' or conditioned medium?
Options
A. It is the media full of growth factors used for the growth of cells
B. It is the medium added to nurse the callus culture
C. Both (a) and (b)
D. It is the liquid medium removed from the suspension of fast growing cells
Show Answer
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Correct Answer: It is the liquid medium removed from the suspension of fast growing cells
6. Concentration of sucrose generally used in plant cell culture media is
Options
A. 10-15 g/l
B. 20-30 g/l
C. 40-50 g/l
D. 60-70 g/l
Show Answer
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Correct Answer: 20-30 g/l
7. Which of the following is not true about nurse or conditioned medium?
Options
A. It is liquid removed from the suspension of fast growing cells
B. It contains uncharacterized growth factor released by growing cells
C. It is used in the culture of regenerating protoplast
D. it is removed aseptically from the culture and is autoclaved before use
Show Answer
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Correct Answer: it is removed aseptically from the culture and is autoclaved before use
8. Which of the following growth regulator is used to stimulate embryo or shoot development?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Discuss
Correct Answer: Gibberellins
9. To maintain the pH of the culture
Options
A. organic acid such as citric, fumaric, malic and succinic acid is used
B. synthetic buffers such as Tris, MES or HEPS are used
C. both (a) and (b)
D. ammonium salts are used
Show Answer
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Correct Answer: both (a) and (b)
10. Starch content of potatoes can be increased by using a bacterial gene, known as
Options
A. sucrose phosphate synthase gene
B. ADP glucose pyrophosphorylase gene
C. polygalactouranase gene
D. none of the above
Show Answer
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Discuss
Correct Answer: ADP glucose pyrophosphorylase gene
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