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Media Required for Plant Cell Culture
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Question
Which is the most common carbon source used in the plant cell culture media?
Options
A. Sucrose
B. Glucose
C. Fructose
D. Maltose
Correct Answer
Sucrose
Media Required for Plant Cell Culture problems
Search Results
1. Which of the following is not an auxin?
Options
A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. zeatin
D. Indole butyric acid
Show Answer
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Discuss
Correct Answer: zeatin
2. Which of the following growth regulator promote cell division?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Discuss
Correct Answer: Cytokinins
3. Silver thiosulphate is added to culture medium as it helps to
Options
A. maintain the pH
B. remove toxic phenolics from plant cells
C. prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
D. all of the above
Show Answer
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Discuss
Correct Answer: prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
4. What is 'nurse' or conditioned medium?
Options
A. It is the media full of growth factors used for the growth of cells
B. It is the medium added to nurse the callus culture
C. Both (a) and (b)
D. It is the liquid medium removed from the suspension of fast growing cells
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Correct Answer: It is the liquid medium removed from the suspension of fast growing cells
5. Very high sugar concentration (40-100 g/l) have been used
Options
A. in specialized secondary metabolite production
B. to adjust the osmotic potential of the media in short term treatment for regeneration
C. Both (a) and (b)
D. none of these
Show Answer
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Discuss
Correct Answer: Both (a) and (b)
6. Which of the following is an ethylene biosynthesis inhibitor?
Options
A. Citric acid
B. Succinic acid
C. Activated charcoal
D. Silver thiosulphate
Show Answer
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Discuss
Correct Answer: Silver thiosulphate
7. Concentration of sucrose generally used in plant cell culture media is
Options
A. 10-15 g/l
B. 20-30 g/l
C. 40-50 g/l
D. 60-70 g/l
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Correct Answer: 20-30 g/l
8. Which of the following is not true about nurse or conditioned medium?
Options
A. It is liquid removed from the suspension of fast growing cells
B. It contains uncharacterized growth factor released by growing cells
C. It is used in the culture of regenerating protoplast
D. it is removed aseptically from the culture and is autoclaved before use
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Correct Answer: it is removed aseptically from the culture and is autoclaved before use
9. Which of the following growth regulator is used to stimulate embryo or shoot development?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
Show Answer
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Discuss
Correct Answer: Gibberellins
10. To maintain the pH of the culture
Options
A. organic acid such as citric, fumaric, malic and succinic acid is used
B. synthetic buffers such as Tris, MES or HEPS are used
C. both (a) and (b)
D. ammonium salts are used
Show Answer
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Correct Answer: both (a) and (b)
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