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Home Biotechnology Media Required for Plant Cell Culture Comments

  • Question
  • Silver thiosulphate is added to culture medium as it helps to


  • Options
  • A. maintain the pH
  • B. remove toxic phenolics from plant cells
  • C. prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
  • D. all of the above

  • Correct Answer
  • prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. 


  • Media Required for Plant Cell Culture problems


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    • 1. What is 'nurse' or conditioned medium?

    • Options
    • A. It is the media full of growth factors used for the growth of cells
    • B. It is the medium added to nurse the callus culture
    • C. Both (a) and (b)
    • D. It is the liquid medium removed from the suspension of fast growing cells
    • Discuss
    • 2. Very high sugar concentration (40-100 g/l) have been used

    • Options
    • A. in specialized secondary metabolite production
    • B. to adjust the osmotic potential of the media in short term treatment for regeneration
    • C. Both (a) and (b)
    • D. none of these
    • Discuss
    • 3. Which of the following growth regulator cause plant cells to grow?

    • Options
    • A. Auxins
    • B. Cytokinins
    • C. Gibberellins
    • D. Brassinosteroids
    • Discuss
    • 4. Which of the following is not a cytokinin?

    • Options
    • A. 2,4-dichlorophenoxyacetic acid
    • B. 6 benzylaminopurine
    • C. Zeatin
    • D. Kinetin
    • Discuss
    • 5. Neutralized activated charcoal is occasionally added to young regenerating cultures to

    • Options
    • A. remove toxic phenolics produced by the stressed plant cell
    • B. help to remove plants growth regulators introduced at an earlier stage
    • C. both (a) and (b)
    • D. maintain the pH of the medium
    • Discuss
    • 6. Which of the following growth regulator promote cell division?

    • Options
    • A. Auxins
    • B. Cytokinins
    • C. Gibberellins
    • D. Brassinosteroids
    • Discuss
    • 7. Which of the following is not an auxin?

    • Options
    • A. Indole acetic acid (IAA)
    • B. Naphthalenacetic acid (NAA)
    • C. zeatin
    • D. Indole butyric acid
    • Discuss
    • 8. Which is the most common carbon source used in the plant cell culture media?

    • Options
    • A. Sucrose
    • B. Glucose
    • C. Fructose
    • D. Maltose
    • Discuss
    • 9. Which of the following is an ethylene biosynthesis inhibitor?

    • Options
    • A. Citric acid
    • B. Succinic acid
    • C. Activated charcoal
    • D. Silver thiosulphate
    • Discuss
    • 10. Concentration of sucrose generally used in plant cell culture media is

    • Options
    • A. 10-15 g/l
    • B. 20-30 g/l
    • C. 40-50 g/l
    • D. 60-70 g/l
    • Discuss


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