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Home Biotechnology Media Required for Plant Cell Culture Comments

  • Question
  • Which of the following growth regulator is added for short initiation during plant regeneration from callus?


  • Options
  • A. Auxins
  • B. Cytokinins
  • C. Gibberellins
  • D. Brassinosteroids

  • Correct Answer
  • Cytokinins 


  • Media Required for Plant Cell Culture problems


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    • 1. Which is/are the naturally occurring plant auxins?

    • Options
    • A. Indole acetic acid (IAA)
    • B. Naphthalenacetic acid (NAA)
    • C. 2,4-dichlorophenoxyacetic acid
    • D. All of the above
    • Discuss
    • 2. What are the macronutrients used in plant cell culture medium?

    • Options
    • A. N, P, K, S, Na
    • B. N, P, K, Ca, Cl
    • C. N, P, K, S, Ca
    • D. N, P, Ca, Na, Cl
    • Discuss
    • 3. Which is/are the disadvantage/(s) of using IAA in plant cell culture media?

    • Options
    • A. It is unstable in solution
    • B. Gets easily oxidized
    • C. Conjugated to inactive form by plant cells
    • D. All of the above
    • Discuss
    • 4. In plant cell culture media, auxins and cytokinins are used in the range of

    • Options
    • A. l-50?M
    • B. 50-100?M
    • C. 100-125?M
    • D. more than 125?M
    • Discuss
    • 5. Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

    • Options
    • A. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
    • B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
    • C. utilization of both ammonium and nitrate result in rise in pH
    • D. utilization of both ammonium and nitrate result in drop in pH
    • Discuss
    • 6. Neutralized activated charcoal is occasionally added to young regenerating cultures to

    • Options
    • A. remove toxic phenolics produced by the stressed plant cell
    • B. help to remove plants growth regulators introduced at an earlier stage
    • C. both (a) and (b)
    • D. maintain the pH of the medium
    • Discuss
    • 7. Which of the following is not a cytokinin?

    • Options
    • A. 2,4-dichlorophenoxyacetic acid
    • B. 6 benzylaminopurine
    • C. Zeatin
    • D. Kinetin
    • Discuss
    • 8. Which of the following growth regulator cause plant cells to grow?

    • Options
    • A. Auxins
    • B. Cytokinins
    • C. Gibberellins
    • D. Brassinosteroids
    • Discuss
    • 9. Very high sugar concentration (40-100 g/l) have been used

    • Options
    • A. in specialized secondary metabolite production
    • B. to adjust the osmotic potential of the media in short term treatment for regeneration
    • C. Both (a) and (b)
    • D. none of these
    • Discuss
    • 10. What is 'nurse' or conditioned medium?

    • Options
    • A. It is the media full of growth factors used for the growth of cells
    • B. It is the medium added to nurse the callus culture
    • C. Both (a) and (b)
    • D. It is the liquid medium removed from the suspension of fast growing cells
    • Discuss


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