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Media Required for Plant Cell Culture
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Question
In plant cell culture media, auxins and cytokinins are used in the range of
Options
A. l-50?M
B. 50-100?M
C. 100-125?M
D. more than 125?M
Correct Answer
l-50?M
Media Required for Plant Cell Culture problems
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1. Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media
Options
A. utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
C. utilization of both ammonium and nitrate result in rise in pH
D. utilization of both ammonium and nitrate result in drop in pH
Show Answer
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Correct Answer: utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
2. When an organism has a life cycle with alternation of generations, the haploid generation is the
Options
A. zygote
B. gametophyte
C. gamete
D. sporophyte
Show Answer
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Correct Answer: gametophyte
3. A plasmid used to introduce BT toxin into a plant cell
Options
A. carries an antibiotic resistance gene that makes the plant resistant to antibiotics
B. causes the plant to produce opines
C. is toxic for the plant cell
D. carries a bacterial gene with a plant promoter
Show Answer
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Correct Answer: carries a bacterial gene with a plant promoter
4. The shortest of mitotic phases is the
Options
A. telophase
B. interphase
C. metaphase
D. anaphase
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Correct Answer: anaphase
5. When F1 plants are crossed with homozygous recessive plants, it is known as
Options
A. test cross
B. recessive cross
C. criss-cross
D. para-cross
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Correct Answer: test cross
6. Which is/are the disadvantage/(s) of using IAA in plant cell culture media?
Options
A. It is unstable in solution
B. Gets easily oxidized
C. Conjugated to inactive form by plant cells
D. All of the above
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Correct Answer: All of the above
7. What are the macronutrients used in plant cell culture medium?
Options
A. N, P, K, S, Na
B. N, P, K, Ca, Cl
C. N, P, K, S, Ca
D. N, P, Ca, Na, Cl
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Correct Answer: N, P, K, S, Ca
8. Which is/are the naturally occurring plant auxins?
Options
A. Indole acetic acid (IAA)
B. Naphthalenacetic acid (NAA)
C. 2,4-dichlorophenoxyacetic acid
D. All of the above
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Correct Answer: Indole acetic acid (IAA)
9. Which of the following growth regulator is added for short initiation during plant regeneration from callus?
Options
A. Auxins
B. Cytokinins
C. Gibberellins
D. Brassinosteroids
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Correct Answer: Cytokinins
10. Neutralized activated charcoal is occasionally added to young regenerating cultures to
Options
A. remove toxic phenolics produced by the stressed plant cell
B. help to remove plants growth regulators introduced at an earlier stage
C. both (a) and (b)
D. maintain the pH of the medium
Show Answer
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Correct Answer: both (a) and (b)
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