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Home Biotechnology Cloning Vectors Comments

  • Question
  • Stuffer is


  • Options
  • A. the right arm of the vector DNA
  • B. the left arm of the vector DNA
  • C. central fragment of the vector DNA
  • D. none of the above

  • Correct Answer
  • central fragment of the vector DNA 


  • Cloning Vectors problems


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    • 1. Single stranded vectors are useful

    • Options
    • A. for sequencing of cloned DNA
    • B. for oligonucleotide directed mutagenesis
    • C. for probe preparation
    • D. all of the above
    • Discuss
    • 2. Cosmid vectors are

    • Options
    • A. plasmids that contain fragment of ? DNA including the cos site
    • B. phages that lack cos site
    • C. plasmids that have no selection marker
    • D. cryptic plasmids
    • Discuss
    • 3. Charon 34 and Charon 35 can clone DNA upto

    • Options
    • A. 1-2 kb
    • B. 6-7 kb
    • C. 9-20 kb
    • D. 30-35 kb
    • Discuss
    • 4. Select the wrong statement about plasmids?

    • Options
    • A. It is extrachromosomal
    • B. It is double stranded
    • C. Its replication depends upon host cell
    • D. It is closed and circular DNA
    • Discuss
    • 5. Maximum size of foreign DNA that can be inserted into a replacement vector is

    • Options
    • A. 25-30 kb
    • B. 18-20 kb
    • C. 20-25 kb
    • D. 40-50 kb
    • Discuss
    • 6. Phagemid consist of

    • Options
    • A. plasmid vector carrying ? phage's cos site
    • B. plasmid vector carrying ? attachment (? att) site
    • C. plasmid vector carrying origin of replication of ? phage only
    • D. plasmid vector carrying origin of replication of plasmid only
    • Discuss
    • 7. P1 cloning vector allow cloning of DNA of the length of

    • Options
    • A. 100 kbp
    • B. 50 kbp
    • C. 20 kbp
    • D. 10 kbp
    • Discuss
    • 8. Who among the following coined the term Biotechnology?

    • Options
    • A. Karl Ereky
    • B. James Clarke
    • C. Paul Terasaky
    • D. Clarke and Sommer
    • Discuss
    • 9. In the bundle sheath of C4 plant __________ is/ are less than mesophyll.

    • Options
    • A. PSI
    • B. PSII
    • C. PSI or PSII
    • D. PSI and PSII
    • Discuss
    • 10. Which group of land plants is most restricted to moist environments?

    • Options
    • A. Lycophyta
    • B. Sphenophyta
    • C. Bryophyta
    • D. Angiosperms
    • Discuss


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