Discussion
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- Question
Cosmid vectors are
Options- A. plasmids that contain fragment of ? DNA including the cos site
- B. phages that lack cos site
- C. plasmids that have no selection marker
- D. cryptic plasmids
- Correct Answer
- plasmids that contain fragment of ? DNA including the cos site
- 1. Charon 34 and Charon 35 can clone DNA upto
Options- A. 1-2 kb
- B. 6-7 kb
- C. 9-20 kb
- D. 30-35 kb Discuss
- 2. Select the wrong statement about plasmids?
Options- A. It is extrachromosomal
- B. It is double stranded
- C. Its replication depends upon host cell
- D. It is closed and circular DNA Discuss
- 3. Maximum size of foreign DNA that can be inserted into a replacement vector is
Options- A. 25-30 kb
- B. 18-20 kb
- C. 20-25 kb
- D. 40-50 kb Discuss
- 4. Plasmids which are maintained as multiple copy number per cell are known as
Options- A. stringent plasmids
- B. relaxed plasmids
- C. cryptic plasmids
- D. none of these Discuss
- 5. Conjugative plasmids
Options- A. exhibit antibiotic resistance
- B. do not exhibit antibiotic resistance
- C. carry transfer genes called the tra genes
- D. do not carry transfer genes Discuss
- 6. Single stranded vectors are useful
Options- A. for sequencing of cloned DNA
- B. for oligonucleotide directed mutagenesis
- C. for probe preparation
- D. all of the above Discuss
- 7. Stuffer is
Options- A. the right arm of the vector DNA
- B. the left arm of the vector DNA
- C. central fragment of the vector DNA
- D. none of the above Discuss
- 8. Phagemid consist of
Options- A. plasmid vector carrying ? phage's cos site
- B. plasmid vector carrying ? attachment (? att) site
- C. plasmid vector carrying origin of replication of ? phage only
- D. plasmid vector carrying origin of replication of plasmid only Discuss
- 9. P1 cloning vector allow cloning of DNA of the length of
Options- A. 100 kbp
- B. 50 kbp
- C. 20 kbp
- D. 10 kbp Discuss
- 10. Who among the following coined the term Biotechnology?
Options- A. Karl Ereky
- B. James Clarke
- C. Paul Terasaky
- D. Clarke and Sommer Discuss
Cloning Vectors problems
Search Results
Correct Answer: 9-20 kb
Correct Answer: Its replication depends upon host cell
Correct Answer: 20-25 kb
Correct Answer: relaxed plasmids
Correct Answer: carry transfer genes called the tra genes
Correct Answer: all of the above
Correct Answer: central fragment of the vector DNA
Correct Answer: plasmid vector carrying ? attachment (? att) site
Correct Answer: 100 kbp
Correct Answer: Karl Ereky
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