Discussion
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- Question
? ZAP vector is an example of
Options- A. phage
- B. phagemid
- C. cosmid
- D. plasmid
- Correct Answer
- phagemid
- 1. Cosmid vectors are used for
Options- A. cloning small fragments of DNA
- B. cloning large fragments of DNA
- C. cloning prokaryotic DNA only
- D. cloning eukaryotic DNA only Discuss
- 2. ? gt 10 and ? gt 11 vectors can propagate cloned fragments up to
Options- A. 6-7 kb
- B. 1-2 kb
- C. 40-44 kb
- D. 20-23 kb Discuss
- 3. Phagemid vectors are
Options- A. combination of plasmid and phage ?
- B. combination of phages and cosmid
- C. phages carrying properties of plasmids
- D. all of the above Discuss
- 4. pBR 322 has/have which of the following selection marker(s)?
Options- A. Ampr
- B. Tetr
- C. Both (a) and (b)
- D. Kanr Discuss
- 5. Difference between ? gt 10 and ? gt 11 vectors is that
Options- A. ? gt 11 is an expression vector
- B. ? gt 10 is an expression vector
- C. ? gt 10 is a replacement vector
- D. ? gt 11 is a replacement vector Discuss
- 6. Cosmids lack
Options- A. genes coding for viral proteins
- B. origin of replication
- C. marker genes coding for replication
- D. cleavage site for the insertion of foreign DNA Discuss
- 7. A plasmid can be considered as a suitable cloning vector if
Options- A. it can be readily isolated from the cells
- B. it possesses a single restriction site for one or more restriction enzymes
- C. insertion of foreign DNA does not alter its replication properties
- D. All of the above Discuss
- 8. Which of the following is not true about phagemid?
Options- A. Contain functional origin of replication of the plasmid and ? phage
- B. May be propagated as a plasmid or as phage in appropriate strain
- C. Contain ? att site
- D. Can only be propagated as phage Discuss
- 9. Size of the DNA that can be packaged into a ? phage is
Options- A. 50 kb
- B. 35-53 kb
- C. 40-50 kb
- D. any size Discuss
- 10. Inserted DNA in ? gt 11 can be expressed as
Options- A. ?-galactosidase fused protein
- B. free protein in the cytoplasm
- C. free protein that is secreted out
- D. all of the above Discuss
Cloning Vectors problems
Search Results
Correct Answer: cloning large fragments of DNA
Correct Answer: 6-7 kb
Correct Answer: combination of plasmid and phage ?
Correct Answer: Both (a) and (b)
Correct Answer: ? gt 11 is an expression vector
Correct Answer: genes coding for viral proteins
Correct Answer: All of the above
Correct Answer: Can only be propagated as phage
Correct Answer: 35-53 kb
Correct Answer: ?-galactosidase fused protein
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