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  • Question
  • ? gt 10 and ? gt 11 vectors can propagate cloned fragments up to


  • Options
  • A. 6-7 kb
  • B. 1-2 kb
  • C. 40-44 kb
  • D. 20-23 kb

  • Correct Answer
  • 6-7 kb 


  • Cloning Vectors problems


    Search Results


    • 1. Phagemid vectors are

    • Options
    • A. combination of plasmid and phage ?
    • B. combination of phages and cosmid
    • C. phages carrying properties of plasmids
    • D. all of the above
    • Discuss
    • 2. pBR 322 has/have which of the following selection marker(s)?

    • Options
    • A. Ampr
    • B. Tetr
    • C. Both (a) and (b)
    • D. Kanr
    • Discuss
    • 3. Difference between ? gt 10 and ? gt 11 vectors is that

    • Options
    • A. ? gt 11 is an expression vector
    • B. ? gt 10 is an expression vector
    • C. ? gt 10 is a replacement vector
    • D. ? gt 11 is a replacement vector
    • Discuss
    • 4. Charon 34 and Charon 35 are the examples of

    • Options
    • A. plasmid vector
    • B. cosmid vector
    • C. phage vector
    • D. phagemid vector
    • Discuss
    • 5. Plasmid incompatibility is

    • Options
    • A. inability of a plasmid to grow in the host
    • B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 6. Cosmid vectors are used for

    • Options
    • A. cloning small fragments of DNA
    • B. cloning large fragments of DNA
    • C. cloning prokaryotic DNA only
    • D. cloning eukaryotic DNA only
    • Discuss
    • 7. ? ZAP vector is an example of

    • Options
    • A. phage
    • B. phagemid
    • C. cosmid
    • D. plasmid
    • Discuss
    • 8. Cosmids lack

    • Options
    • A. genes coding for viral proteins
    • B. origin of replication
    • C. marker genes coding for replication
    • D. cleavage site for the insertion of foreign DNA
    • Discuss
    • 9. A plasmid can be considered as a suitable cloning vector if

    • Options
    • A. it can be readily isolated from the cells
    • B. it possesses a single restriction site for one or more restriction enzymes
    • C. insertion of foreign DNA does not alter its replication properties
    • D. All of the above
    • Discuss
    • 10. Which of the following is not true about phagemid?

    • Options
    • A. Contain functional origin of replication of the plasmid and ? phage
    • B. May be propagated as a plasmid or as phage in appropriate strain
    • C. Contain ? att site
    • D. Can only be propagated as phage
    • Discuss


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