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Discussion
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Biotechnology
‣
Cloning Vectors
Comments
Question
? gt 10 and ? gt 11 vectors can propagate cloned fragments up to
Options
A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb
Correct Answer
6-7 kb
Cloning Vectors problems
Search Results
1. Phagemid vectors are
Options
A. combination of plasmid and phage ?
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: combination of plasmid and phage ?
2. pBR 322 has/have which of the following selection marker(s)?
Options
A. Amp
r
B. Tet
r
C. Both (a) and (b)
D. Kan
r
Show Answer
Scratch Pad
Discuss
Correct Answer: Both (a) and (b)
3. Difference between ? gt 10 and ? gt 11 vectors is that
Options
A. ? gt 11 is an expression vector
B. ? gt 10 is an expression vector
C. ? gt 10 is a replacement vector
D. ? gt 11 is a replacement vector
Show Answer
Scratch Pad
Discuss
Correct Answer: ? gt 11 is an expression vector
4. Charon 34 and Charon 35 are the examples of
Options
A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector
Show Answer
Scratch Pad
Discuss
Correct Answer: phage vector
5. Plasmid incompatibility is
Options
A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
6. Cosmid vectors are used for
Options
A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only
Show Answer
Scratch Pad
Discuss
Correct Answer: cloning large fragments of DNA
7. ? ZAP vector is an example of
Options
A. phage
B. phagemid
C. cosmid
D. plasmid
Show Answer
Scratch Pad
Discuss
Correct Answer: phagemid
8. Cosmids lack
Options
A. genes coding for viral proteins
B. origin of replication
C. marker genes coding for replication
D. cleavage site for the insertion of foreign DNA
Show Answer
Scratch Pad
Discuss
Correct Answer: genes coding for viral proteins
9. A plasmid can be considered as a suitable cloning vector if
Options
A. it can be readily isolated from the cells
B. it possesses a single restriction site for one or more restriction enzymes
C. insertion of foreign DNA does not alter its replication properties
D. All of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: All of the above
10. Which of the following is not true about phagemid?
Options
A. Contain functional origin of replication of the plasmid and ? phage
B. May be propagated as a plasmid or as phage in appropriate strain
C. Contain ? att site
D. Can only be propagated as phage
Show Answer
Scratch Pad
Discuss
Correct Answer: Can only be propagated as phage
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