Discussion
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- Question
Phagemid vectors are
Options- A. combination of plasmid and phage ?
- B. combination of phages and cosmid
- C. phages carrying properties of plasmids
- D. all of the above
- Correct Answer
- combination of plasmid and phage ?
- 1. pBR 322 has/have which of the following selection marker(s)?
Options- A. Ampr
- B. Tetr
- C. Both (a) and (b)
- D. Kanr Discuss
- 2. Difference between ? gt 10 and ? gt 11 vectors is that
Options- A. ? gt 11 is an expression vector
- B. ? gt 10 is an expression vector
- C. ? gt 10 is a replacement vector
- D. ? gt 11 is a replacement vector Discuss
- 3. Charon 34 and Charon 35 are the examples of
Options- A. plasmid vector
- B. cosmid vector
- C. phage vector
- D. phagemid vector Discuss
- 4. Plasmid incompatibility is
Options- A. inability of a plasmid to grow in the host
- B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
- C. both (a) and (b)
- D. none of the above Discuss
- 5. M 13 is an example of
Options- A. filamentous phage
- B. single stranded DNA vector
- C. both (a) and (b)
- D. plasmid Discuss
- 6. ? gt 10 and ? gt 11 vectors can propagate cloned fragments up to
Options- A. 6-7 kb
- B. 1-2 kb
- C. 40-44 kb
- D. 20-23 kb Discuss
- 7. Cosmid vectors are used for
Options- A. cloning small fragments of DNA
- B. cloning large fragments of DNA
- C. cloning prokaryotic DNA only
- D. cloning eukaryotic DNA only Discuss
- 8. ? ZAP vector is an example of
Options- A. phage
- B. phagemid
- C. cosmid
- D. plasmid Discuss
- 9. Cosmids lack
Options- A. genes coding for viral proteins
- B. origin of replication
- C. marker genes coding for replication
- D. cleavage site for the insertion of foreign DNA Discuss
- 10. A plasmid can be considered as a suitable cloning vector if
Options- A. it can be readily isolated from the cells
- B. it possesses a single restriction site for one or more restriction enzymes
- C. insertion of foreign DNA does not alter its replication properties
- D. All of the above Discuss
Cloning Vectors problems
Search Results
Correct Answer: Both (a) and (b)
Correct Answer: ? gt 11 is an expression vector
Correct Answer: phage vector
Correct Answer: inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
Correct Answer: both (a) and (b)
Correct Answer: 6-7 kb
Correct Answer: cloning large fragments of DNA
Correct Answer: phagemid
Correct Answer: genes coding for viral proteins
Correct Answer: All of the above
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