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Biotechnology
‣
Cloning Vectors
Comments
Question
Charon 34 and Charon 35 are the examples of
Options
A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector
Correct Answer
phage vector
Cloning Vectors problems
Search Results
1. Plasmid incompatibility is
Options
A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
2. M 13 is an example of
Options
A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid
Show Answer
Scratch Pad
Discuss
Correct Answer: both (a) and (b)
3. P1 cloning vector is the example of
Options
A. plasmid
B. cosmid
C. bacteriophage
D. phagemid
Show Answer
Scratch Pad
Discuss
Correct Answer: bacteriophage
4. Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as
Options
A. source of nutrients
B. carbon source
C. antifoam agent
D. both (a) and (c)
Show Answer
Scratch Pad
Discuss
Correct Answer: antifoam agent
5. Which of the following event occurs during second phase of growth of
P. chrysogenum
?
Options
A. Synthesis of penicillin is high
B. Mycelial mass increases
C. pH increase
D. Both (a) and (b)
Show Answer
Scratch Pad
Discuss
Correct Answer: Both (a) and (b)
6. Difference between ? gt 10 and ? gt 11 vectors is that
Options
A. ? gt 11 is an expression vector
B. ? gt 10 is an expression vector
C. ? gt 10 is a replacement vector
D. ? gt 11 is a replacement vector
Show Answer
Scratch Pad
Discuss
Correct Answer: ? gt 11 is an expression vector
7. pBR 322 has/have which of the following selection marker(s)?
Options
A. Amp
r
B. Tet
r
C. Both (a) and (b)
D. Kan
r
Show Answer
Scratch Pad
Discuss
Correct Answer: Both (a) and (b)
8. Phagemid vectors are
Options
A. combination of plasmid and phage ?
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: combination of plasmid and phage ?
9. ? gt 10 and ? gt 11 vectors can propagate cloned fragments up to
Options
A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb
Show Answer
Scratch Pad
Discuss
Correct Answer: 6-7 kb
10. Cosmid vectors are used for
Options
A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only
Show Answer
Scratch Pad
Discuss
Correct Answer: cloning large fragments of DNA
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