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Home Biotechnology Cloning Vectors Comments

  • Question
  • M 13 is an example of


  • Options
  • A. filamentous phage
  • B. single stranded DNA vector
  • C. both (a) and (b)
  • D. plasmid

  • Correct Answer
  • both (a) and (b) 


  • Cloning Vectors problems


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    • 1. P1 cloning vector is the example of

    • Options
    • A. plasmid
    • B. cosmid
    • C. bacteriophage
    • D. phagemid
    • Discuss
    • 2. Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as

    • Options
    • A. source of nutrients
    • B. carbon source
    • C. antifoam agent
    • D. both (a) and (c)
    • Discuss
    • 3. Which of the following event occurs during second phase of growth of P. chrysogenum?

    • Options
    • A. Synthesis of penicillin is high
    • B. Mycelial mass increases
    • C. pH increase
    • D. Both (a) and (b)
    • Discuss
    • 4. Which of the following event occurs during third phase of growth of P chrysogenum?

    • Options
    • A. Concentration of antibiotic increases in the medium
    • B. Autolysis of the medium starts
    • C. Slight rise in pH due to liberation of ammonia
    • D. All of the above
    • Discuss
    • 5. High yield of chlorotetracyclin requires

    • Options
    • A. no aeration
    • B. continuous aeration
    • C. aeration which does not affect the yield
    • D. controlled aeration
    • Discuss
    • 6. Plasmid incompatibility is

    • Options
    • A. inability of a plasmid to grow in the host
    • B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 7. Charon 34 and Charon 35 are the examples of

    • Options
    • A. plasmid vector
    • B. cosmid vector
    • C. phage vector
    • D. phagemid vector
    • Discuss
    • 8. Difference between ? gt 10 and ? gt 11 vectors is that

    • Options
    • A. ? gt 11 is an expression vector
    • B. ? gt 10 is an expression vector
    • C. ? gt 10 is a replacement vector
    • D. ? gt 11 is a replacement vector
    • Discuss
    • 9. pBR 322 has/have which of the following selection marker(s)?

    • Options
    • A. Ampr
    • B. Tetr
    • C. Both (a) and (b)
    • D. Kanr
    • Discuss
    • 10. Phagemid vectors are

    • Options
    • A. combination of plasmid and phage ?
    • B. combination of phages and cosmid
    • C. phages carrying properties of plasmids
    • D. all of the above
    • Discuss


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