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Biotechnology
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Vectors Uses for Animal Cell Culture
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Question
Which of the following promoters is repressed in presence of glucose?
Options
A. Galactokinase (GAL 1)
B. Acid phosphatase (PHOS)
C. Both (a) and (b)
D. Phosphoglycerate kinase (PGK)
Correct Answer
Galactokinase (GAL 1)
Vectors Uses for Animal Cell Culture problems
Search Results
1. Maximum amount of foreign DNA introduced into SV 40 viruses is
Options
A. 10 kb
B. 5 kb
C. 2.5 kb
D. 1 kb
Show Answer
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Discuss
Correct Answer: 2.5 kb
2. Autonomously replicating sequence is not present in
Options
A. YIp
B. YRp
C. YCp
D. YAC
Show Answer
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Discuss
Correct Answer: YIp
3. Which of the following can be used for reducing the size of gene inserts containing introns?
Options
A. Vaccinia virus
B. Baculo virus
C. Retro virus
D. none of these
Show Answer
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Discuss
Correct Answer: Retro virus
4. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
Options
A. Contains centromeric sequences
B. Segregates during meiosis and mitosis
C. Functions as true chromosome
D. All of the above
Show Answer
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Correct Answer: All of the above
5. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Options
A. Allows transformation by using crossing over
B. Has a origin of replication and
rep
genes
C. Carries autonomously replicating sequence
D. Carries centromere and telomere sequences
Show Answer
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Correct Answer: Allows transformation by using crossing over
6. In case of Baculoviruses, the foreign gene
Options
A. is inserted downstream of polyhedrin promoter
B. is inserted downstream of viral origin of replication
C. is inserted between the polyhedrin promoter and downstream sequences
D. is inserted randomly in viral genome
Show Answer
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Discuss
Correct Answer: is inserted between the polyhedrin promoter and downstream sequences
7. Bovine papilloma virus has a circular double stranded DNA genome of about
Options
A. 2.5 kb
B. 5.2 kb
C. 7.9 kb
D. 9.5 kb
Show Answer
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Correct Answer: 7.9 kb
8. Which of the following viruses are used as vectors for transferring genes to animal cells?
Options
A. SV 40 virus
B. Bovine papilloma virus (BVP)
C. Retroviruses
D. All of these
Show Answer
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Correct Answer: All of these
9. Moloney-Mu-leukemia virus is an example of
Options
A. Vaccinia virus
B. Baculo virus
C. Retro virus
D. none of these
Show Answer
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Discuss
Correct Answer: Retro virus
10. Cloning genes in the yeast offer which of following advantage(s)?
Options
A. Over production of proteins of commercial value
B. Ability to glycosylate proteins during secretion
C. Ability to clone large piece of DNA
D. All of the above
Show Answer
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Discuss
Correct Answer: All of the above
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