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Biotechnology
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Vectors Uses for Animal Cell Culture
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Question
Autonomously replicating sequence is not present in
Options
A. YIp
B. YRp
C. YCp
D. YAC
Correct Answer
YIp
Vectors Uses for Animal Cell Culture problems
Search Results
1. Which of the following can be used for reducing the size of gene inserts containing introns?
Options
A. Vaccinia virus
B. Baculo virus
C. Retro virus
D. none of these
Show Answer
Scratch Pad
Discuss
Correct Answer: Retro virus
2. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
Options
A. Contains centromeric sequences
B. Segregates during meiosis and mitosis
C. Functions as true chromosome
D. All of the above
Show Answer
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Discuss
Correct Answer: All of the above
3. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Options
A. Allows transformation by using crossing over
B. Has a origin of replication and
rep
genes
C. Carries autonomously replicating sequence
D. Carries centromere and telomere sequences
Show Answer
Scratch Pad
Discuss
Correct Answer: Allows transformation by using crossing over
4. SV 40 vectors, in which early region replacements are carried out, require
Options
A. COS cell line
B. SV 40 helper virus
C. pBR 322 vector
D. none of these
Show Answer
Scratch Pad
Discuss
Correct Answer: COS cell line
5. Which one of the following is the most stable vector?
Options
A. YEp
B. YRp
C. YAC
D. YIp
Show Answer
Scratch Pad
Discuss
Correct Answer: YAC
6. Maximum amount of foreign DNA introduced into SV 40 viruses is
Options
A. 10 kb
B. 5 kb
C. 2.5 kb
D. 1 kb
Show Answer
Scratch Pad
Discuss
Correct Answer: 2.5 kb
7. Which of the following promoters is repressed in presence of glucose?
Options
A. Galactokinase (GAL 1)
B. Acid phosphatase (PHOS)
C. Both (a) and (b)
D. Phosphoglycerate kinase (PGK)
Show Answer
Scratch Pad
Discuss
Correct Answer: Galactokinase (GAL 1)
8. In case of Baculoviruses, the foreign gene
Options
A. is inserted downstream of polyhedrin promoter
B. is inserted downstream of viral origin of replication
C. is inserted between the polyhedrin promoter and downstream sequences
D. is inserted randomly in viral genome
Show Answer
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Discuss
Correct Answer: is inserted between the polyhedrin promoter and downstream sequences
9. Bovine papilloma virus has a circular double stranded DNA genome of about
Options
A. 2.5 kb
B. 5.2 kb
C. 7.9 kb
D. 9.5 kb
Show Answer
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Discuss
Correct Answer: 7.9 kb
10. Which of the following viruses are used as vectors for transferring genes to animal cells?
Options
A. SV 40 virus
B. Bovine papilloma virus (BVP)
C. Retroviruses
D. All of these
Show Answer
Scratch Pad
Discuss
Correct Answer: All of these
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