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- Question
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?
Options- A. Contains centromeric sequences
- B. Segregates during meiosis and mitosis
- C. Functions as true chromosome
- D. All of the above
- Correct Answer
- All of the above
- 1. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
- 2. SV 40 vectors, in which early region replacements are carried out, require
Options- A. COS cell line
- B. SV 40 helper virus
- C. pBR 322 vector
- D. none of these Discuss
- 3. Which one of the following is the most stable vector?
Options- A. YEp
- B. YRp
- C. YAC
- D. YIp Discuss
- 4. Yeast episomal plasmids (YEp) were constructed using
Options- A. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- B. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
- C. 12?m yeast plasmid and E coli vector pMB 9
- D. 12?m yeast plasmid and E coli vector pBR 322 Discuss
- 5. Cos cell line is
Options- A. cohesive end site of phage ?
- B. derivative of permissive CV-1 monkey cell line
- C. cohesive initial site of phage ?
- D. derivative of non-permissive CV-1 monkey cell line Discuss
- 6. Which of the following can be used for reducing the size of gene inserts containing introns?
Options- A. Vaccinia virus
- B. Baculo virus
- C. Retro virus
- D. none of these Discuss
- 7. Autonomously replicating sequence is not present in
Options- A. YIp
- B. YRp
- C. YCp
- D. YAC Discuss
- 8. Maximum amount of foreign DNA introduced into SV 40 viruses is
Options- A. 10 kb
- B. 5 kb
- C. 2.5 kb
- D. 1 kb Discuss
- 9. Which of the following promoters is repressed in presence of glucose?
Options- A. Galactokinase (GAL 1)
- B. Acid phosphatase (PHOS)
- C. Both (a) and (b)
- D. Phosphoglycerate kinase (PGK) Discuss
- 10. In case of Baculoviruses, the foreign gene
Options- A. is inserted downstream of polyhedrin promoter
- B. is inserted downstream of viral origin of replication
- C. is inserted between the polyhedrin promoter and downstream sequences
- D. is inserted randomly in viral genome Discuss
Vectors Uses for Animal Cell Culture problems
Search Results
Correct Answer: Allows transformation by using crossing over
Correct Answer: COS cell line
Correct Answer: YAC
Correct Answer: 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
Correct Answer: derivative of permissive CV-1 monkey cell line
Correct Answer: Retro virus
Correct Answer: YIp
Correct Answer: 2.5 kb
Correct Answer: Galactokinase (GAL 1)
Correct Answer: is inserted between the polyhedrin promoter and downstream sequences
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