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Home Biotechnology Vectors Uses for Animal Cell Culture Comments

  • Question
  • SV 40 vectors, in which early region replacements are carried out, require


  • Options
  • A. COS cell line
  • B. SV 40 helper virus
  • C. pBR 322 vector
  • D. none of these

  • Correct Answer
  • COS cell line 


  • Vectors Uses for Animal Cell Culture problems


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    • 1. Which one of the following is the most stable vector?

    • Options
    • A. YEp
    • B. YRp
    • C. YAC
    • D. YIp
    • Discuss
    • 2. Yeast episomal plasmids (YEp) were constructed using

    • Options
    • A. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
    • B. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
    • C. 12?m yeast plasmid and E coli vector pMB 9
    • D. 12?m yeast plasmid and E coli vector pBR 322
    • Discuss
    • 3. Cos cell line is

    • Options
    • A. cohesive end site of phage ?
    • B. derivative of permissive CV-1 monkey cell line
    • C. cohesive initial site of phage ?
    • D. derivative of non-permissive CV-1 monkey cell line
    • Discuss
    • 4. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

    • Options
    • A. transcriptional regulators and are essential for viral replication
    • B. not necessary for viral replication
    • C. coding for virus coat protein
    • D. important for packaging of viral DNA into viral head
    • Discuss
    • 5. Yeast Replicating plasmids (YRp) carry

    • Options
    • A. centromere
    • B. telomere
    • C. autonomously replicating sequence
    • D. both (a) and (c)
    • Discuss
    • 6. Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

    • Options
    • A. Allows transformation by using crossing over
    • B. Has a origin of replication and rep genes
    • C. Carries autonomously replicating sequence
    • D. Carries centromere and telomere sequences
    • Discuss
    • 7. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

    • Options
    • A. Contains centromeric sequences
    • B. Segregates during meiosis and mitosis
    • C. Functions as true chromosome
    • D. All of the above
    • Discuss
    • 8. Which of the following can be used for reducing the size of gene inserts containing introns?

    • Options
    • A. Vaccinia virus
    • B. Baculo virus
    • C. Retro virus
    • D. none of these
    • Discuss
    • 9. Autonomously replicating sequence is not present in

    • Options
    • A. YIp
    • B. YRp
    • C. YCp
    • D. YAC
    • Discuss
    • 10. Maximum amount of foreign DNA introduced into SV 40 viruses is

    • Options
    • A. 10 kb
    • B. 5 kb
    • C. 2.5 kb
    • D. 1 kb
    • Discuss


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