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- Question
Yeast Replicating plasmids (YRp) carry
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. both (a) and (c)
- Correct Answer
- autonomously replicating sequence
- 1. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
- 2. Retroviruses have advantage for being used as vector for animal cells because
Options- A. they cover a wide host range
- B. infection does not lead to cell death, infected cells produce virus over an indefinite period
- C. viral gene expression is driven by strong promoters
- D. all of the above Discuss
- 3. The size of the genome of SV 40 is
Options- A. 7.2 kb
- B. 69 kb
- C. 5.2 kb
- D. 110 kb Discuss
- 4. Which of the following(s) is/are the most commonly used markers in yeast?
Options- A. His 3
- B. Ura 3
- C. Trp 1
- D. All of these Discuss
- 5. Recombinant SV40 viruses introduce the foreign DNA into cells
Options- A. with the DNA mediated transfection
- B. without the DNA mediated transfection
- C. with the RNA mediated transfection
- D. without the RNA mediated transfection Discuss
- 6. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are
Options- A. transcriptional regulators and are essential for viral replication
- B. not necessary for viral replication
- C. coding for virus coat protein
- D. important for packaging of viral DNA into viral head Discuss
- 7. Cos cell line is
Options- A. cohesive end site of phage ?
- B. derivative of permissive CV-1 monkey cell line
- C. cohesive initial site of phage ?
- D. derivative of non-permissive CV-1 monkey cell line Discuss
- 8. Yeast episomal plasmids (YEp) were constructed using
Options- A. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
- B. 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
- C. 12?m yeast plasmid and E coli vector pMB 9
- D. 12?m yeast plasmid and E coli vector pBR 322 Discuss
- 9. Which one of the following is the most stable vector?
Options- A. YEp
- B. YRp
- C. YAC
- D. YIp Discuss
- 10. SV 40 vectors, in which early region replacements are carried out, require
Options- A. COS cell line
- B. SV 40 helper virus
- C. pBR 322 vector
- D. none of these Discuss
Vectors Uses for Animal Cell Culture problems
Search Results
Correct Answer: Has a origin of replication and rep genes
Correct Answer: all of the above
Correct Answer: 5.2 kb
Correct Answer: All of these
Correct Answer: without the DNA mediated transfection
Correct Answer: transcriptional regulators and are essential for viral replication
Correct Answer: derivative of permissive CV-1 monkey cell line
Correct Answer: 2?m yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
Correct Answer: YAC
Correct Answer: COS cell line
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