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Home ‣ Biotechnology ‣ Vectors Uses for Animal Cell Culture Comments
  • Question

  • Which of the following(s) is/are the most commonly used markers in yeast?


  • Options
  • A. His 3
  • B. Ura 3
  • C. Trp 1
  • D. All of these

  • Correct Answer
  • All of these 

    • Vectors Uses for Animal Cell Culture problems

    Search Results

    • 1. Recombinant SV40 viruses introduce the foreign DNA into cells

    • Options
    • A. with the DNA mediated transfection
    • B. without the DNA mediated transfection
    • C. with the RNA mediated transfection
    • D. without the RNA mediated transfection
      • Discuss
    • 2. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

    • Options
    • A. episomally only
    • B. integrated only
    • C. either episomally or integrated at high copy number
    • D. either episomally or integrated at low copy number
      • Discuss
    • 3. Maximum insert size in case of adenovirus vectors is

    • Options
    • A. 6-8 kb
    • B. 4-6 kb
    • C. 10-12 kb
    • D. 20 kb
      • Discuss
    • 4. Which of the following are produced in high copy number?

    • Options
    • A. YEp
    • B. YRp
    • C. Both (a) and (b)
    • D. YCp
      • Discuss
    • 5. YAC vectors can be used to obtain artificial chromosomes because it carries

    • Options
    • A. centromere
    • B. telomere
    • C. autonomously replicating sequence
    • D. all of these
      • Discuss
    • 6. The size of the genome of SV 40 is

    • Options
    • A. 7.2 kb
    • B. 69 kb
    • C. 5.2 kb
    • D. 110 kb
      • Discuss
    • 7. Retroviruses have advantage for being used as vector for animal cells because

    • Options
    • A. they cover a wide host range
    • B. infection does not lead to cell death, infected cells produce virus over an indefinite period
    • C. viral gene expression is driven by strong promoters
    • D. all of the above
      • Discuss
    • 8. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

    • Options
    • A. Allows transformation by using crossing over
    • B. Has a origin of replication and rep genes
    • C. Carries autonomously replicating sequence
    • D. Carries centromere and telomere sequences
      • Discuss
    • 9. Yeast Replicating plasmids (YRp) carry

    • Options
    • A. centromere
    • B. telomere
    • C. autonomously replicating sequence
    • D. both (a) and (c)
      • Discuss
    • 10. Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

    • Options
    • A. transcriptional regulators and are essential for viral replication
    • B. not necessary for viral replication
    • C. coding for virus coat protein
    • D. important for packaging of viral DNA into viral head
      • Discuss

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