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Home ‣ Biotechnology ‣ Vectors Uses for Animal Cell Culture Comments
- Question
Recombinant SV40 viruses introduce the foreign DNA into cells
Options- A. with the DNA mediated transfection
- B. without the DNA mediated transfection
- C. with the RNA mediated transfection
- D. without the RNA mediated transfection
- Correct Answer
- without the DNA mediated transfection
- 1. Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA
Options- A. episomally only
- B. integrated only
- C. either episomally or integrated at high copy number
- D. either episomally or integrated at low copy number Discuss
- 2. Maximum insert size in case of adenovirus vectors is
Options- A. 6-8 kb
- B. 4-6 kb
- C. 10-12 kb
- D. 20 kb Discuss
- 3. Which of the following are produced in high copy number?
Options- A. YEp
- B. YRp
- C. Both (a) and (b)
- D. YCp Discuss
- 4. YAC vectors can be used to obtain artificial chromosomes because it carries
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. all of these Discuss
- 5. Lytic infection of SV 40 does not occur in
Options- A. permissive cells
- B. non permissive cells
- C. both (a) and (b)
- D. none of these Discuss
- 6. Which of the following(s) is/are the most commonly used markers in yeast?
Options- A. His 3
- B. Ura 3
- C. Trp 1
- D. All of these Discuss
- 7. The size of the genome of SV 40 is
Options- A. 7.2 kb
- B. 69 kb
- C. 5.2 kb
- D. 110 kb Discuss
- 8. Retroviruses have advantage for being used as vector for animal cells because
Options- A. they cover a wide host range
- B. infection does not lead to cell death, infected cells produce virus over an indefinite period
- C. viral gene expression is driven by strong promoters
- D. all of the above Discuss
- 9. Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?
Options- A. Allows transformation by using crossing over
- B. Has a origin of replication and rep genes
- C. Carries autonomously replicating sequence
- D. Carries centromere and telomere sequences Discuss
- 10. Yeast Replicating plasmids (YRp) carry
Options- A. centromere
- B. telomere
- C. autonomously replicating sequence
- D. both (a) and (c) Discuss
Vectors Uses for Animal Cell Culture problems
Search Results
Correct Answer: either episomally or integrated at high copy number
Correct Answer: 6-8 kb
Correct Answer: Both (a) and (b)
Correct Answer: all of these
Correct Answer: non permissive cells
Correct Answer: All of these
Correct Answer: 5.2 kb
Correct Answer: all of the above
Correct Answer: Has a origin of replication and rep genes
Correct Answer: autonomously replicating sequence
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