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- Question
Which of the following is not true about the helper plasmids?
Options- A. These can replicate in Agrobacterium
- B. These help in the mediating conjugation of intermediate vectors
- C. These can't replicate in Agrobacterium
- D. All of the above
- Correct Answer
- These can replicate in Agrobacterium
- 1. Microprojectile method of gene transfer in plants involves delivery of DNA
Options- A. with the help of micromanipulator
- B. with the help of bolistics
- C. with the help of needles
- D. any of the above Discuss
- 2. Co-integrating transformation vectors must include a region of homology in
Options- A. the vector plasmid
- B. the Ti-plasmid
- C. between vector plasmid and Ti-plasmid
- D. none of these Discuss
- 3. Agrobacterium tumefaciens is a
Options- A. gram (+) bacteria
- B. gram (-) bacteria
- C. a fungi
- D. a yeast Discuss
- 4. Opines are
Options- A. amino acid derivatives found in tumor tissues
- B. amino acid derivatives found in normal tissues
- C. amino acid derivatives found in both normal as well as tumor tissues
- D. none of the above Discuss
- 5. Advantage of microprojectile method over microinjection method for gene transfer in plants include
Options- A. intact cells are used
- B. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
- C. gene can be transferred to many cells simultaneously
- D. all of the above Discuss
- 6. Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
Options- A. pRK 2013
- B. pRN 3
- C. either (a) or (b)
- D. none of these Discuss
- 7. Microinjection involves
Options- A. injection of large amount of DNA
- B. injection of DNA into bigger cells
- C. injection with needle having diameter greater than cell diameter
- D. all of the above Discuss
- 8. Which of the following is true about T DNA?
Options- A. Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
- B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
- C. Integration of T DNA occurs at two specific sites in the plant nuclear DNA
- D. Integration of T DNA occurs at one site that may be random in the plant nuclear DNA Discuss
- 9. Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
Options- A. one set of sequence necessary for gene transfer
- B. two sets of sequence necessary for gene transfer
- C. three sets of sequence necessary for gene transfer
- D. four sets of sequence necessary for gene transfer Discuss
- 10. Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
Options- A. pBR 322 based plasmid vector
- B. pRK 2013
- C. pRN 3
- D. all of these Discuss
Gene Transfer in Plants problems
Search Results
Correct Answer: with the help of bolistics
Correct Answer: between vector plasmid and Ti-plasmid
Correct Answer: gram (-) bacteria
Correct Answer: amino acid derivatives found in tumor tissues
Correct Answer: all of the above
Correct Answer: either (a) or (b)
Correct Answer: injection with needle having diameter greater than cell diameter
Correct Answer: Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Correct Answer: two sets of sequence necessary for gene transfer
Correct Answer: pBR 322 based plasmid vector
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