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- Question
Agrobacterium tumefaciens is a
Options- A. gram (+) bacteria
- B. gram (-) bacteria
- C. a fungi
- D. a yeast
- Correct Answer
- gram (-) bacteria
- 1. Opines are
Options- A. amino acid derivatives found in tumor tissues
- B. amino acid derivatives found in normal tissues
- C. amino acid derivatives found in both normal as well as tumor tissues
- D. none of the above Discuss
- 2. Advantage of microprojectile method over microinjection method for gene transfer in plants include
Options- A. intact cells are used
- B. method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall
- C. gene can be transferred to many cells simultaneously
- D. all of the above Discuss
- 3. The size of the virulent plasmid of Agrobacterium tumefaciens is
Options- A. 40-80 kb
- B. 80-120 kb
- C. 140-235 kb
- D. >235 kb Discuss
- 4. Plant transformation vectors based on Agrobacterium can generally be divided into
Options- A. two vectors
- B. four vectors
- C. six vectors
- D. eight vectors Discuss
- 5. The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
Options- A. cos site
- B. flanking sequences
- C. border sequences
- D. transfer sequences Discuss
- 6. Co-integrating transformation vectors must include a region of homology in
Options- A. the vector plasmid
- B. the Ti-plasmid
- C. between vector plasmid and Ti-plasmid
- D. none of these Discuss
- 7. Microprojectile method of gene transfer in plants involves delivery of DNA
Options- A. with the help of micromanipulator
- B. with the help of bolistics
- C. with the help of needles
- D. any of the above Discuss
- 8. Which of the following is not true about the helper plasmids?
Options- A. These can replicate in Agrobacterium
- B. These help in the mediating conjugation of intermediate vectors
- C. These can't replicate in Agrobacterium
- D. All of the above Discuss
- 9. Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
Options- A. pRK 2013
- B. pRN 3
- C. either (a) or (b)
- D. none of these Discuss
- 10. Microinjection involves
Options- A. injection of large amount of DNA
- B. injection of DNA into bigger cells
- C. injection with needle having diameter greater than cell diameter
- D. all of the above Discuss
Gene Transfer in Plants problems
Search Results
Correct Answer: amino acid derivatives found in tumor tissues
Correct Answer: all of the above
Correct Answer: 140-235 kb
Correct Answer: two vectors
Correct Answer: border sequences
Correct Answer: between vector plasmid and Ti-plasmid
Correct Answer: with the help of bolistics
Correct Answer: These can replicate in Agrobacterium
Correct Answer: either (a) or (b)
Correct Answer: injection with needle having diameter greater than cell diameter
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