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Biotechnology
‣
Gene Transfer in Plants
Comments
Question
Direct DNA uptake by protoplasts can be stimulated by
Options
A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. all of these
Correct Answer
polyethylene glycol (PEG)
Gene Transfer in Plants problems
Search Results
1. T-DNA transfer and processing into plant genome requires products of which of the following genes?
Options
A.
vir
A,B
B.
vir
G,C
C.
vir
D,E
D. All of these
Show Answer
Scratch Pad
Discuss
Correct Answer: All of these
2. The transfer of intermediate vectors into
Agrobacterium
are brought about by
Options
A. transformation
B. biparental mating
C. triparental mating
D. transduction
Show Answer
Scratch Pad
Discuss
Correct Answer: triparental mating
3. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
Options
A.
vir
A
B.
vir
B
C.
vir
C
D.
vir
D
Show Answer
Scratch Pad
Discuss
Correct Answer:
vir
D
4. Opine synthesis is the property
Options
A. conferred to plant cells when it transformed by
Agrobacterium tumefaciens
B. determined by the bacteria
Agrobacterium tumefaciens
C. both (a) and (b)
D. of normal plant cells
Show Answer
Scratch Pad
Discuss
Correct Answer: both (a) and (b)
5. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
Options
A.
vir
A and
vir
G
B.
vir
C and
vir
D
C.
vir
B and
vir
E
D.
vir
A and
vir
B
Show Answer
Scratch Pad
Discuss
Correct Answer:
vir
A and
vir
G
6. Liposomes mediated gene transfer in plants involves
Options
A. plasmid DNA enclosed in a lipid bag
B. fusion of liposomes with protoplast
C. use of polyethylene glycol (PEG)
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: all of the above
7. The left segment of octopine T-DNA (TL) is necessary for
Options
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Show Answer
Scratch Pad
Discuss
Correct Answer: tumour formation
8. Integrated nopaline T-DNA occurs as
Options
A. single segment
B. two segments
C. three segments
D. four segments
Show Answer
Scratch Pad
Discuss
Correct Answer: single segment
9. In the liposome mediated gene transfer in plants, nucleic acids are
Options
A. protected from nuclease digestion
B. stable in liposomes
C. both (a) and (b)
D. not stable in liposomes
Show Answer
Scratch Pad
Discuss
Correct Answer: both (a) and (b)
10. Which of the following is not true about the direct repeats flanking T-DNA?
Options
A. They are conserved between nopaline and octopine Ti-plasmids
B. These repeats are transferred intact to the plant genome
C. These are important in integration mechanism
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: These repeats are transferred intact to the plant genome
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