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Home Biotechnology Gene Transfer in Plants Comments

  • Question
  • Direct DNA uptake by protoplasts can be stimulated by


  • Options
  • A. polyethylene glycol (PEG)
  • B. decanal
  • C. luciferin
  • D. all of these

  • Correct Answer
  • polyethylene glycol (PEG) 


  • Gene Transfer in Plants problems


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    • 1. T-DNA transfer and processing into plant genome requires products of which of the following genes?

    • Options
    • A. vir A,B
    • B. vir G,C
    • C. vir D,E
    • D. All of these
    • Discuss
    • 2. The transfer of intermediate vectors into Agrobacterium are brought about by

    • Options
    • A. transformation
    • B. biparental mating
    • C. triparental mating
    • D. transduction
    • Discuss
    • 3. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

    • Options
    • A. vir A
    • B. vir B
    • C. vir C
    • D. vir D
    • Discuss
    • 4. Opine synthesis is the property

    • Options
    • A. conferred to plant cells when it transformed by Agrobacterium tumefaciens
    • B. determined by the bacteria Agrobacterium tumefaciens
    • C. both (a) and (b)
    • D. of normal plant cells
    • Discuss
    • 5. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?

    • Options
    • A. vir A and vir G
    • B. vir C and vir D
    • C. vir B and vir E
    • D. vir A and vir B
    • Discuss
    • 6. Liposomes mediated gene transfer in plants involves

    • Options
    • A. plasmid DNA enclosed in a lipid bag
    • B. fusion of liposomes with protoplast
    • C. use of polyethylene glycol (PEG)
    • D. all of the above
    • Discuss
    • 7. The left segment of octopine T-DNA (TL) is necessary for

    • Options
    • A. enzymes for agropine biosynthesis
    • B. tumour formation
    • C. conjugative transfer
    • D. all of these
    • Discuss
    • 8. Integrated nopaline T-DNA occurs as

    • Options
    • A. single segment
    • B. two segments
    • C. three segments
    • D. four segments
    • Discuss
    • 9. In the liposome mediated gene transfer in plants, nucleic acids are

    • Options
    • A. protected from nuclease digestion
    • B. stable in liposomes
    • C. both (a) and (b)
    • D. not stable in liposomes
    • Discuss
    • 10. Which of the following is not true about the direct repeats flanking T-DNA?

    • Options
    • A. They are conserved between nopaline and octopine Ti-plasmids
    • B. These repeats are transferred intact to the plant genome
    • C. These are important in integration mechanism
    • D. all of the above
    • Discuss


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