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- Question
Which of the following is true about Agrobacterium tumefaciens?
Options- A. It causes crown gall disease of plants
- B. It infects gymosperms
- C. It infects dicotyledonous angiosperms
- D. All of the above
- Correct Answer
- All of the above
- 1. Which technique is used to introduce genes into dicots?
Options- A. electroporation
- B. particle acceleration
- C. microinjection
- D. Ti plasmid infection Discuss
- 2. Opines that are present in crown gall tumour include
Options- A. octopine
- B. nopaline
- C. agropine
- D. all of these Discuss
- 3. The right segment of octopine T-DNA (TR) is necessary for
Options- A. enzymes for agropine biosynthesis
- B. tumour formation
- C. conjugative transfer
- D. all of these Discuss
- 4. Which of the following are used as selection marker for the cells transformed with Agrobacterium?
Options- A. Neomycin phosphotransferase
- B. Streptomycin phosphotransferase
- C. Hygromycin phosphotransferase
- D. any of the above Discuss
- 5. Virulence trait of Agrobacterium tumefaciens is borne on
Options- A. chromosomal DNA
- B. tumour inducing plasmid DNA
- C. both chromosomal and plasmid DNA
- D. cryptic plasmid DNA Discuss
- 6. On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
Options- A. 12 bp
- B. 20 bp
- C. 25 bp
- D. 30 bp Discuss
- 7. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
Options- A. vir A and vir G
- B. vir C and vir D
- C. vir B and vir E
- D. vir A and vir B Discuss
- 8. Opine synthesis is the property
Options- A. conferred to plant cells when it transformed by Agrobacterium tumefaciens
- B. determined by the bacteria Agrobacterium tumefaciens
- C. both (a) and (b)
- D. of normal plant cells Discuss
- 9. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
Options- A. vir A
- B. vir B
- C. vir C
- D. vir D Discuss
- 10. The transfer of intermediate vectors into Agrobacterium are brought about by
Options- A. transformation
- B. biparental mating
- C. triparental mating
- D. transduction Discuss
Gene Transfer in Plants problems
Search Results
Correct Answer: Ti plasmid infection
Correct Answer: all of these
Correct Answer: enzymes for agropine biosynthesis
Correct Answer: any of the above
Correct Answer: tumour inducing plasmid DNA
Correct Answer: 25 bp
Correct Answer: vir A and vir G
Correct Answer: both (a) and (b)
Correct Answer: vir D
Correct Answer: triparental mating
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