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Discussion
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‣
Biotechnology
‣
Gene Transfer in Plants
Comments
Question
Which technique is used to introduce genes into dicots?
Options
A. electroporation
B. particle acceleration
C. microinjection
D. Ti plasmid infection
Correct Answer
Ti plasmid infection
Gene Transfer in Plants problems
Search Results
1. Opines that are present in crown gall tumour include
Options
A. octopine
B. nopaline
C. agropine
D. all of these
Show Answer
Scratch Pad
Discuss
Correct Answer: all of these
2. The right segment of octopine T-DNA (TR) is necessary for
Options
A. enzymes for agropine biosynthesis
B. tumour formation
C. conjugative transfer
D. all of these
Show Answer
Scratch Pad
Discuss
Correct Answer: enzymes for agropine biosynthesis
3. Which of the following are used as selection marker for the cells transformed with
Agrobacterium
?
Options
A. Neomycin phosphotransferase
B. Streptomycin phosphotransferase
C. Hygromycin phosphotransferase
D. any of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: any of the above
4. Virulence trait of
Agrobacterium tumefaciens
is borne on
Options
A. chromosomal DNA
B. tumour inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA
Show Answer
Scratch Pad
Discuss
Correct Answer: tumour inducing plasmid DNA
5. Vir genes required for the T-DNA transfer and processing are located
Options
A. on the T-DNA
B. outside the T-DNA region
C. on the plant genome
D. none of these
Show Answer
Scratch Pad
Discuss
Correct Answer: outside the T-DNA region
6. Which of the following is true about
Agrobacterium tumefaciens
?
Options
A. It causes crown gall disease of plants
B. It infects gymosperms
C. It infects dicotyledonous angiosperms
D. All of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: All of the above
7. On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
Options
A. 12 bp
B. 20 bp
C. 25 bp
D. 30 bp
Show Answer
Scratch Pad
Discuss
Correct Answer: 25 bp
8. Which of the following genes are constitutively expressed and control the plant induced activation of other vir genes?
Options
A.
vir
A and
vir
G
B.
vir
C and
vir
D
C.
vir
B and
vir
E
D.
vir
A and
vir
B
Show Answer
Scratch Pad
Discuss
Correct Answer:
vir
A and
vir
G
9. Opine synthesis is the property
Options
A. conferred to plant cells when it transformed by
Agrobacterium tumefaciens
B. determined by the bacteria
Agrobacterium tumefaciens
C. both (a) and (b)
D. of normal plant cells
Show Answer
Scratch Pad
Discuss
Correct Answer: both (a) and (b)
10. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
Options
A.
vir
A
B.
vir
B
C.
vir
C
D.
vir
D
Show Answer
Scratch Pad
Discuss
Correct Answer:
vir
D
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