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  • Question
  • Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?


  • Options
  • A. Nurse medium
  • B. Nurse or feeder culture
  • C. Both (a) and (b)
  • D. None of these

  • Correct Answer
  • Both (a) and (b) 


  • Plant Cell Culture problems


    Search Results


    • 1. A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.

    • Options
    • A. microshoot
    • B. medium
    • C. explant
    • D. scion
    • Discuss
    • 2. Organogenesis is

    • Options
    • A. formation of callus tissue
    • B. formation of root and shoots on callus tissue
    • C. both (a) and (b)
    • D. genesis of organs
    • Discuss
    • 3. What is meant by 'Organ culture'?

    • Options
    • A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
    • B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
    • C. Cultivation of organs in a laboratory through the synthesis of tissues
    • D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
    • Discuss
    • 4. In a callus culture

    • Options
    • A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
    • B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
    • C. auxins and cytokinins are not required
    • D. only auxin is required for root and shoot formation
    • Discuss
    • 5. Agrobacterium based gene transfer is efficient

    • Options
    • A. only with dicots
    • B. only with monocots
    • C. with both monocots and dicots
    • D. with majority monocots and few dicots
    • Discuss
    • 6. Protoplasts are the cells devoid of

    • Options
    • A. cell membrane
    • B. cell wall
    • C. both cell wall and cell membrane
    • D. none of these
    • Discuss
    • 7. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?

    • Options
    • A. Mass selection
    • B. Protoplast fusion
    • C. Transformation
    • D. Transpiration
    • Discuss
    • 8. Cellular totipotency is the property of

    • Options
    • A. plants
    • B. animals
    • C. bacteria
    • D. all of these
    • Discuss
    • 9. The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

    • Options
    • A. redifferentiation
    • B. dedifferentiation
    • C. either (a) or (b)
    • D. none of these
    • Discuss
    • 10. Integrated octopine T DNA occurs as

    • Options
    • A. single segment
    • B. two segments
    • C. three segments
    • D. four segments
    • Discuss


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