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Biotechnology
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Plant Cell Culture
Comments
Question
In a callus culture
Options
A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C. auxins and cytokinins are not required
D. only auxin is required for root and shoot formation
Correct Answer
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
Plant Cell Culture problems
Search Results
1. Agrobacterium based gene transfer is efficient
Options
A. only with dicots
B. only with monocots
C. with both monocots and dicots
D. with majority monocots and few dicots
Show Answer
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Discuss
Correct Answer: only with dicots
2. What is/are the benefit(s) of micropropagation or clonal propagation?
Options
A. Rapid multiplication of superior clones
B. Multiplication of disease free plants
C. Multiplication of sexually derived sterile hybrids
D. All of the above
Show Answer
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Correct Answer: All of the above
3. Subculturing is similar to propagation by cuttings because
Options
A. it separates multiple microshoots and places them in a medium
B. it uses scions to produce new microshoots
C. they both use in vitro growing conditions
D. all of the above
Show Answer
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Correct Answer: it separates multiple microshoots and places them in a medium
4. Which method of plant propagation involves the use of girdling?
Options
A. Grafting
B. Cuttings
C. Layering
D. Micropropagation
Show Answer
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Discuss
Correct Answer: Layering
5. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
Options
A. 2-5 days
B. 5-10 days
C. 10-15 days
D. 15-17 days
Show Answer
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Correct Answer: 5-10 days
6. What is meant by 'Organ culture'?
Options
A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C. Cultivation of organs in a laboratory through the synthesis of tissues
D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
Show Answer
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Correct Answer: Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
7. Organogenesis is
Options
A. formation of callus tissue
B. formation of root and shoots on callus tissue
C. both (a) and (b)
D. genesis of organs
Show Answer
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Discuss
Correct Answer: formation of root and shoots on callus tissue
8. A(
n
) __________ is an excised piece of leaf or stem tissue used in micropropagation.
Options
A. microshoot
B. medium
C. explant
D. scion
Show Answer
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Correct Answer: explant
9. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
Options
A. Nurse medium
B. Nurse or feeder culture
C. Both (a) and (b)
D. None of these
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Correct Answer: Both (a) and (b)
10. Protoplasts are the cells devoid of
Options
A. cell membrane
B. cell wall
C. both cell wall and cell membrane
D. none of these
Show Answer
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Discuss
Correct Answer: cell wall
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