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Biotechnology
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Plant Cell Culture
Comments
Question
Which method of plant propagation involves the use of girdling?
Options
A. Grafting
B. Cuttings
C. Layering
D. Micropropagation
Correct Answer
Layering
Plant Cell Culture problems
Search Results
1. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
Options
A. 2-5 days
B. 5-10 days
C. 10-15 days
D. 15-17 days
Show Answer
Scratch Pad
Discuss
Correct Answer: 5-10 days
2. The ability of the component cells of callus to form a whole plant is known as
Options
A. redifferentiation
B. dedifferentiation
C. either (a) or (b)
D. none of these
Show Answer
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Discuss
Correct Answer: redifferentiation
3. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
Options
A. cellulotyic enzymes
B. pectolytic enzymes
C. both cellulotyic and pectolytic enzymes
D. proteolytic enzymes
Show Answer
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Discuss
Correct Answer: both cellulotyic and pectolytic enzymes
4. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
Options
A. Multiplication of sexually derived sterile hybrids
B. Less multiplication of disease free plants
C. Storage and transportation of propogates
D. Both (b) and (c)
Show Answer
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Discuss
Correct Answer: Storage and transportation of propogates
5. Cell fusion method includes the preparation of large number of
Options
A. plant cells stripped of their cell wall
B. single plant cell stripped of their cell wall
C. plant cells with cell wall
D. cells from different species
Show Answer
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Discuss
Correct Answer: single plant cell stripped of their cell wall
6. Subculturing is similar to propagation by cuttings because
Options
A. it separates multiple microshoots and places them in a medium
B. it uses scions to produce new microshoots
C. they both use in vitro growing conditions
D. all of the above
Show Answer
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Discuss
Correct Answer: it separates multiple microshoots and places them in a medium
7. What is/are the benefit(s) of micropropagation or clonal propagation?
Options
A. Rapid multiplication of superior clones
B. Multiplication of disease free plants
C. Multiplication of sexually derived sterile hybrids
D. All of the above
Show Answer
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Discuss
Correct Answer: All of the above
8. Agrobacterium based gene transfer is efficient
Options
A. only with dicots
B. only with monocots
C. with both monocots and dicots
D. with majority monocots and few dicots
Show Answer
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Discuss
Correct Answer: only with dicots
9. In a callus culture
Options
A. increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C. auxins and cytokinins are not required
D. only auxin is required for root and shoot formation
Show Answer
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Discuss
Correct Answer: increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
10. What is meant by 'Organ culture'?
Options
A. Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C. Cultivation of organs in a laboratory through the synthesis of tissues
D. The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
Show Answer
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Discuss
Correct Answer: Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
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