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  • Question
  • Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with


  • Options
  • A. cellulotyic enzymes
  • B. pectolytic enzymes
  • C. both cellulotyic and pectolytic enzymes
  • D. proteolytic enzymes

  • Correct Answer
  • both cellulotyic and pectolytic enzymes 


  • Plant Cell Culture problems


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    • 1. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

    • Options
    • A. Multiplication of sexually derived sterile hybrids
    • B. Less multiplication of disease free plants
    • C. Storage and transportation of propogates
    • D. Both (b) and (c)
    • Discuss
    • 2. Cell fusion method includes the preparation of large number of

    • Options
    • A. plant cells stripped of their cell wall
    • B. single plant cell stripped of their cell wall
    • C. plant cells with cell wall
    • D. cells from different species
    • Discuss
    • 3. Phloem is composed of

    • Options
    • A. vessel elements and companion cells
    • B. tracheids and vessel elements
    • C. sieve-tube elements and companion cells
    • D. tracheids and sieve-tube elements
    • Discuss
    • 4. The evaporation of water from leaves is called

    • Options
    • A. transpiration
    • B. cohesion
    • C. tension
    • D. pressure flow
    • Discuss
    • 5. The cohesion-tension theory of water movement helps to explain how water moves from soil into root?

    • Options
    • A. Through the phloem
    • B. From leaf to root
    • C. Through the xylem
    • D. Into the guard cells
    • Discuss
    • 6. The ability of the component cells of callus to form a whole plant is known as

    • Options
    • A. redifferentiation
    • B. dedifferentiation
    • C. either (a) or (b)
    • D. none of these
    • Discuss
    • 7. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

    • Options
    • A. 2-5 days
    • B. 5-10 days
    • C. 10-15 days
    • D. 15-17 days
    • Discuss
    • 8. Which method of plant propagation involves the use of girdling?

    • Options
    • A. Grafting
    • B. Cuttings
    • C. Layering
    • D. Micropropagation
    • Discuss
    • 9. Subculturing is similar to propagation by cuttings because

    • Options
    • A. it separates multiple microshoots and places them in a medium
    • B. it uses scions to produce new microshoots
    • C. they both use in vitro growing conditions
    • D. all of the above
    • Discuss
    • 10. What is/are the benefit(s) of micropropagation or clonal propagation?

    • Options
    • A. Rapid multiplication of superior clones
    • B. Multiplication of disease free plants
    • C. Multiplication of sexually derived sterile hybrids
    • D. All of the above
    • Discuss


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