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Home Biotechnology Synthesis of Therapeutic Agents Comments

  • Question
  • Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of


  • Options
  • A. ?-Galactosidase
  • B. galactokinase
  • C. acid phosphatase
  • D. glucokinase

  • Correct Answer
  • ?-Galactosidase 


  • Synthesis of Therapeutic Agents problems


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    • 1. Which type of restriction endonuclease cuts the DNA within the recognition site?

    • Options
    • A. Type I
    • B. Type II
    • C. Type III
    • D. All of the these
    • Discuss
    • 2. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

    • Options
    • A. laser
    • B. DNA probe
    • C. gene gun
    • D. inoculating needle
    • Discuss
    • 3. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

    • Options
    • A. photoreactivation
    • B. gel electrophoresis
    • C. autoradiography
    • D. blotting
    • Discuss
    • 4. For gene probes to be useful they must

    • Options
    • A. be large enough to contain gene-specific sequences
    • B. be labeled in some manner to allow detection
    • C. both (a) and (b)
    • D. none of the above
    • Discuss
    • 5. The advantage of using DNA polymerases from thermophilic organisms in PCR is that

    • Options
    • A. the DNA polymerases of these bacteria are much faster than those from other organisms
    • B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
    • C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
    • D. all of the above
    • Discuss
    • 6. The promoter used for cloning HBV surface antigen is

    • Options
    • A. galactokinase
    • B. alcohol dehydrogenase 1
    • C. ?-galactosidase
    • D. polyhedrin
    • Discuss
    • 7. Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen?

    • Options
    • A. Viral core antigen (HBcAg)
    • B. Viral surface antigen (HBsAg)
    • C. e antigen (HBeAg)
    • D. All of the above
    • Discuss
    • 8. Foot and mouth disease (FMD) of animals is caused by

    • Options
    • A. RNA virus
    • B. DNA virus
    • C. bacteria
    • D. protozoa
    • Discuss
    • 9. In India first genetically engineered vaccine against HBV was developed by

    • Options
    • A. Ranbaxy Pvt Ltd
    • B. Shantha Biotechnics Pvt Ltd
    • C. Dabur Pvt Ltd
    • D. Glaxo Pvt Ltd
    • Discuss
    • 10. The first therapeutic product formed by means of recombinant DNA technology is

    • Options
    • A. human growth hormone
    • B. insulin
    • C. hepatitis B vaccine
    • D. vaccine for foot and mouth disease
    • Discuss


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