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- Question
The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
Options- A. laser
- B. DNA probe
- C. gene gun
- D. inoculating needle
- Correct Answer
- gene gun
- 1. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
Options- A. photoreactivation
- B. gel electrophoresis
- C. autoradiography
- D. blotting Discuss
- 2. For gene probes to be useful they must
Options- A. be large enough to contain gene-specific sequences
- B. be labeled in some manner to allow detection
- C. both (a) and (b)
- D. none of the above Discuss
- 3. The advantage of using DNA polymerases from thermophilic organisms in PCR is that
Options- A. the DNA polymerases of these bacteria are much faster than those from other organisms
- B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
- C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
- D. all of the above Discuss
- 4. What is the normal role of restriction endonucleases in bacterial cells?
Options- A. To degrade the bacterial chromosome into small pieces during replication
- B. To degrade invading phage DNA
- C. To produce RNA primers for replication
- D. All of the above Discuss
- 5. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
Options- A. genetic engineering
- B. population genetics
- C. microbiology
- D. protein engineering Discuss
- 6. Which type of restriction endonuclease cuts the DNA within the recognition site?
Options- A. Type I
- B. Type II
- C. Type III
- D. All of the these Discuss
- 7. Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of
Options- A. ?-Galactosidase
- B. galactokinase
- C. acid phosphatase
- D. glucokinase Discuss
- 8. The promoter used for cloning HBV surface antigen is
Options- A. galactokinase
- B. alcohol dehydrogenase 1
- C. ?-galactosidase
- D. polyhedrin Discuss
- 9. Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen?
Options- A. Viral core antigen (HBcAg)
- B. Viral surface antigen (HBsAg)
- C. e antigen (HBeAg)
- D. All of the above Discuss
- 10. Foot and mouth disease (FMD) of animals is caused by
Options- A. RNA virus
- B. DNA virus
- C. bacteria
- D. protozoa Discuss
Recombinant DNA problems
Search Results
Correct Answer: gel electrophoresis
Correct Answer: both (a) and (b)
Correct Answer: the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
Correct Answer: To degrade invading phage DNA
Correct Answer: genetic engineering
Correct Answer: Type II
Correct Answer: ?-Galactosidase
Correct Answer: alcohol dehydrogenase 1
Correct Answer: Viral surface antigen (HBsAg)
Correct Answer: RNA virus
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