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Home Biotechnology Recombinant DNA Comments

  • Question
  • For gene probes to be useful they must


  • Options
  • A. be large enough to contain gene-specific sequences
  • B. be labeled in some manner to allow detection
  • C. both (a) and (b)
  • D. none of the above

  • Correct Answer
  • both (a) and (b) 


  • Recombinant DNA problems


    Search Results


    • 1. The advantage of using DNA polymerases from thermophilic organisms in PCR is that

    • Options
    • A. the DNA polymerases of these bacteria are much faster than those from other organisms
    • B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
    • C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
    • D. all of the above
    • Discuss
    • 2. What is the normal role of restriction endonucleases in bacterial cells?

    • Options
    • A. To degrade the bacterial chromosome into small pieces during replication
    • B. To degrade invading phage DNA
    • C. To produce RNA primers for replication
    • D. All of the above
    • Discuss
    • 3. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

    • Options
    • A. genetic engineering
    • B. population genetics
    • C. microbiology
    • D. protein engineering
    • Discuss
    • 4. Which of the following vector can maintain the largest fragment of foreign DNA?

    • Options
    • A. YAC
    • B. Cosmid
    • C. Plasmid
    • D. Phage
    • Discuss
    • 5. In genetic engineering, a chimera is

    • Options
    • A. an enzyme that links DNA molecules
    • B. a plasmid that contains foreign DNA
    • C. a virus that infects bacteria
    • D. a fungi
    • Discuss
    • 6. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

    • Options
    • A. photoreactivation
    • B. gel electrophoresis
    • C. autoradiography
    • D. blotting
    • Discuss
    • 7. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

    • Options
    • A. laser
    • B. DNA probe
    • C. gene gun
    • D. inoculating needle
    • Discuss
    • 8. Which type of restriction endonuclease cuts the DNA within the recognition site?

    • Options
    • A. Type I
    • B. Type II
    • C. Type III
    • D. All of the these
    • Discuss
    • 9. Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of

    • Options
    • A. ?-Galactosidase
    • B. galactokinase
    • C. acid phosphatase
    • D. glucokinase
    • Discuss
    • 10. The promoter used for cloning HBV surface antigen is

    • Options
    • A. galactokinase
    • B. alcohol dehydrogenase 1
    • C. ?-galactosidase
    • D. polyhedrin
    • Discuss


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