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- Question
The advantage of using DNA polymerases from thermophilic organisms in PCR is that
Options- A. the DNA polymerases of these bacteria are much faster than those from other organisms
- B. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
- C. the DNA polymerases of these bacteria never make mistakes while replicating DNA
- D. all of the above
- Correct Answer
- the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
- 1. What is the normal role of restriction endonucleases in bacterial cells?
Options- A. To degrade the bacterial chromosome into small pieces during replication
- B. To degrade invading phage DNA
- C. To produce RNA primers for replication
- D. All of the above Discuss
- 2. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
Options- A. genetic engineering
- B. population genetics
- C. microbiology
- D. protein engineering Discuss
- 3. Which of the following vector can maintain the largest fragment of foreign DNA?
Options- A. YAC
- B. Cosmid
- C. Plasmid
- D. Phage Discuss
- 4. In genetic engineering, a chimera is
Options- A. an enzyme that links DNA molecules
- B. a plasmid that contains foreign DNA
- C. a virus that infects bacteria
- D. a fungi Discuss
- 5. The Southern blotting technique depends on
Options- A. similarities between the sequences of probe DNA and experimental DNA
- B. similarities between the sequences of probe RNA and experimental RNA
- C. similarities between the sequences of probe protein and experimental protein
- D. the molecular mass of proteins Discuss
- 6. For gene probes to be useful they must
Options- A. be large enough to contain gene-specific sequences
- B. be labeled in some manner to allow detection
- C. both (a) and (b)
- D. none of the above Discuss
- 7. Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
Options- A. photoreactivation
- B. gel electrophoresis
- C. autoradiography
- D. blotting Discuss
- 8. The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
Options- A. laser
- B. DNA probe
- C. gene gun
- D. inoculating needle Discuss
- 9. Which type of restriction endonuclease cuts the DNA within the recognition site?
Options- A. Type I
- B. Type II
- C. Type III
- D. All of the these Discuss
- 10. Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of
Options- A. ?-Galactosidase
- B. galactokinase
- C. acid phosphatase
- D. glucokinase Discuss
Recombinant DNA problems
Search Results
Correct Answer: To degrade invading phage DNA
Correct Answer: genetic engineering
Correct Answer: YAC
Correct Answer: a plasmid that contains foreign DNA
Correct Answer: similarities between the sequences of probe DNA and experimental DNA
Correct Answer: both (a) and (b)
Correct Answer: gel electrophoresis
Correct Answer: gene gun
Correct Answer: Type II
Correct Answer: ?-Galactosidase
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