Discussion
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- Question
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
Options- A. southern blotting
- B. northern blotting
- C. polymerase chain reaction
- D. DNA replication
- Correct Answer
- polymerase chain reaction
- 1. A short molecule containing 2-20 nucleotide is
Options- A. plasmid
- B. vector
- C. oligonucleotide
- D. mononucleotide Discuss
- 2. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
Options- A. a chimera
- B. a transgenic animal
- C. a vector
- D. an enzyme that links DNA molecules Discuss
- 3. Which of the following is not commonly used as vector?
Options- A. Artificial chromosome
- B. Cosmid
- C. Fungi
- D. Plasmid Discuss
- 4. Electroporation is
Options- A. the process of separating charged molecules through a gel maintained in an electric field
- B. the process of combining foreign DNA to an electrically charged vector molecule
- C. the application of high voltage pulses
- D. the process of multiplication of the cells Discuss
- 5. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
Options- A. DNA ligase
- B. helicases
- C. restriction endonucleases
- D. DNA gyrase Discuss
- 6. The Southern blotting technique depends on
Options- A. similarities between the sequences of probe DNA and experimental DNA
- B. similarities between the sequences of probe RNA and experimental RNA
- C. similarities between the sequences of probe protein and experimental protein
- D. the molecular mass of proteins Discuss
- 7. In genetic engineering, a chimera is
Options- A. an enzyme that links DNA molecules
- B. a plasmid that contains foreign DNA
- C. a virus that infects bacteria
- D. a fungi Discuss
- 8. Which of the following vector can maintain the largest fragment of foreign DNA?
Options- A. YAC
- B. Cosmid
- C. Plasmid
- D. Phage Discuss
- 9. The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
Options- A. genetic engineering
- B. population genetics
- C. microbiology
- D. protein engineering Discuss
- 10. What is the normal role of restriction endonucleases in bacterial cells?
Options- A. To degrade the bacterial chromosome into small pieces during replication
- B. To degrade invading phage DNA
- C. To produce RNA primers for replication
- D. All of the above Discuss
Recombinant DNA problems
Search Results
Correct Answer: oligonucleotide
Correct Answer: a transgenic animal
Correct Answer: Fungi
Correct Answer: the application of high voltage pulses
Correct Answer: restriction endonucleases
Correct Answer: similarities between the sequences of probe DNA and experimental DNA
Correct Answer: a plasmid that contains foreign DNA
Correct Answer: YAC
Correct Answer: genetic engineering
Correct Answer: To degrade invading phage DNA
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