Discussion
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- Question
Electroporation is
Options- A. the process of separating charged molecules through a gel maintained in an electric field
- B. the process of combining foreign DNA to an electrically charged vector molecule
- C. the application of high voltage pulses
- D. the process of multiplication of the cells
- Correct Answer
- the application of high voltage pulses
- 1. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
Options- A. DNA ligase
- B. helicases
- C. restriction endonucleases
- D. DNA gyrase Discuss
- 2. Vectors are
Options- A. molecules that degrade nucleic acids
- B. molecules that help in replication
- C. molecules that are able to covalently bond to and carry foreign DNA into cells
- D. molecules that protect host cells from invasion by foreign DNA Discuss
- 3. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
Options- A. DNA polymerase
- B. Restriction endonuclease
- C. DNA ligase
- D. DNA helicase Discuss
- 4. Which of the following is obtained using processed mRNA molecules as a template?
Options- A. rDNA
- B. mDNA
- C. cDNA
- D. tDNA Discuss
- 5. A genomic library is
Options- A. a database where the sequence of an organism's genome is stored
- B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
- C. a book that describes how to isolate DNA from a particular organism
- D. a place where the information of the genetic organization of organisms are kept. Discuss
- 6. Which of the following is not commonly used as vector?
Options- A. Artificial chromosome
- B. Cosmid
- C. Fungi
- D. Plasmid Discuss
- 7. An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
Options- A. a chimera
- B. a transgenic animal
- C. a vector
- D. an enzyme that links DNA molecules Discuss
- 8. A short molecule containing 2-20 nucleotide is
Options- A. plasmid
- B. vector
- C. oligonucleotide
- D. mononucleotide Discuss
- 9. A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
Options- A. southern blotting
- B. northern blotting
- C. polymerase chain reaction
- D. DNA replication Discuss
- 10. The Southern blotting technique depends on
Options- A. similarities between the sequences of probe DNA and experimental DNA
- B. similarities between the sequences of probe RNA and experimental RNA
- C. similarities between the sequences of probe protein and experimental protein
- D. the molecular mass of proteins Discuss
Recombinant DNA problems
Search Results
Correct Answer: restriction endonucleases
Correct Answer: molecules that are able to covalently bond to and carry foreign DNA into cells
Correct Answer: DNA ligase
Correct Answer: cDNA
Correct Answer: a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
Correct Answer: Fungi
Correct Answer: a transgenic animal
Correct Answer: oligonucleotide
Correct Answer: polymerase chain reaction
Correct Answer: similarities between the sequences of probe DNA and experimental DNA
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