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Biotechnology
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Recombinant DNA
Comments
Question
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
Options
A. BACS
B. expression vectors
C. YACS
D. all of these
Correct Answer
expression vectors
Recombinant DNA problems
Search Results
1.
Agrobacterium tumefaciens
is
Options
A. a disease in humans that causes loss of sight
B. a bacterium that can be used to introduce DNA into plants
C. a fungi that is used to produce antibiotics in large amounts
D. a disease in humans that causes loss of weight
Show Answer
Scratch Pad
Discuss
Correct Answer: a bacterium that can be used to introduce DNA into plants
2. Bacterial cells protect their own DNA from degradation by restriction endonucleases by
Options
A. methylating the DNA at the sites that the enzyme recognizes
B. deleting all recognition sites from the genome
C. not producing any restriction endonucleases
D. having anti restriction endonucleases
Show Answer
Scratch Pad
Discuss
Correct Answer: methylating the DNA at the sites that the enzyme recognizes
3. A genomic library is
Options
A. a database where the sequence of an organism's genome is stored
B. a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C. a book that describes how to isolate DNA from a particular organism
D. a place where the information of the genetic organization of organisms are kept.
Show Answer
Scratch Pad
Discuss
Correct Answer: a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
4. Which of the following is obtained using processed mRNA molecules as a template?
Options
A. rDNA
B. mDNA
C. cDNA
D. tDNA
Show Answer
Scratch Pad
Discuss
Correct Answer: cDNA
5. Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
Options
A. DNA polymerase
B. Restriction endonuclease
C. DNA ligase
D. DNA helicase
Show Answer
Scratch Pad
Discuss
Correct Answer: DNA ligase
6. Vectors are
Options
A. molecules that degrade nucleic acids
B. molecules that help in replication
C. molecules that are able to covalently bond to and carry foreign DNA into cells
D. molecules that protect host cells from invasion by foreign DNA
Show Answer
Scratch Pad
Discuss
Correct Answer: molecules that are able to covalently bond to and carry foreign DNA into cells
7. Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
Options
A. DNA ligase
B. helicases
C. restriction endonucleases
D. DNA gyrase
Show Answer
Scratch Pad
Discuss
Correct Answer: restriction endonucleases
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