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Home ‣ Microbiology ‣ DNA Sequencing, Mutation and Repair Comments
- Question
Frame shift mutation may occur as a result of
Options- A. formation of a thymine-dimer
- B. deamination of cytosine to uracil
- C. conversion of guanine to xanthine
- D. none of the above
- Correct Answer
- none of the above
- 1. The promoter on an expression vector used to overproduce proteins in bacteria is
Options- A. a bacterial promoter
- B. expressed both in bacteria and mammalian cells
- C. not a regulated promoter
- D. the natural promoter of the gene being cloned Discuss
- 2. A nonsense mutation may result into
Options- A. an abnormal elongation of a polypeptide
- B. a large deletion within the reading frame of a gene
- C. a premature termination of the synthesis of a polypeptide
- D. modification of mRNA Discuss
- 3. Thymine dimers are directly repaired with the help of visible light by process known as
Options- A. phosphorylation
- B. excision repair
- C. photosynthesis
- D. photoreactivation Discuss
- 4. The location of first hexameric sequence typically found in pro-karyotic promoters is
Options- A. at the transcription start site
- B. approximately 35 bases upstream of the transcription start site
- C. approximately 10 bases upstream of the transcription start site
- D. approximately 25 bases upstream of the transcription start site Discuss
- 5. Which of these catalyzes the synthesis of RNA from a DNA template?
Options- A. DNA gyrase
- B. DNA ligase
- C. DNA polymerase
- D. RNA polymerase Discuss
- 6. Which of the following bacterial genome was sequenced first?
Options- A. E. coli
- B. S. pneumoniae
- C. H. influenzae
- D. S. thermophilus Discuss
- 7. What was the first eukaryotic chromosome to be sequenced?
Options- A. Yeast chromosome III
- B. Yeast chromosome XI
- C. Arabidopsis chromosome IV
- D. None of these Discuss
- 8. The DNA sequences of rRNA genes can be used to
Options- A. assess relatedness of eukaryotes but not prokaryotes
- B. obtain a tentative identification of a new microorganism
- C. predict the pulsed field gel pattern of a microorganism
- D. predict whether a bacteria will be gram-positive or gram-negative Discuss
- 9. The mutation which will not affect the length of a protein is,
Options- A. nonsense mutation
- B. missense mutation
- C. frameshift mutation
- D. all of these Discuss
- 10. Due to which of the following DNA repair mechanisms, one is able to distinguish newly synthesized DNA strands from older one?
Options- A. New strands do not contain cytosine bases
- B. New strands are lower in molecular size
- C. Old strands are methylated while new strands are not
- D. New strands are methylated while old strands are not Discuss
DNA Sequencing, Mutation and Repair problems
Search Results
Correct Answer: a bacterial promoter
Correct Answer: a premature termination of the synthesis of a polypeptide
Correct Answer: photoreactivation
Correct Answer: approximately 35 bases upstream of the transcription start site
Correct Answer: RNA polymerase
Correct Answer: H. influenzae
Correct Answer: Yeast chromosome III
Correct Answer: obtain a tentative identification of a new microorganism
Correct Answer: missense mutation
Correct Answer: Old strands are methylated while new strands are not
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