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  • Question
  • The location of first hexameric sequence typically found in pro-karyotic promoters is


  • Options
  • A. at the transcription start site
  • B. approximately 35 bases upstream of the transcription start site
  • C. approximately 10 bases upstream of the transcription start site
  • D. approximately 25 bases upstream of the transcription start site

  • Correct Answer
  • approximately 35 bases upstream of the transcription start site 


  • Transcription problems


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    • 1. Which of these catalyzes the synthesis of RNA from a DNA template?

    • Options
    • A. DNA gyrase
    • B. DNA ligase
    • C. DNA polymerase
    • D. RNA polymerase
    • Discuss
    • 2. A specific __________ factor helps RNA polymerase bind to late promoters and transcribe late genes.

    • Options
    • A. sigma
    • B. beta
    • C. delta
    • D. gamma
    • Discuss
    • 3. The sigma factor

    • Options
    • A. is responsible for recognizing the termination sequence
    • B. provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis
    • C. recognizes the promotor
    • D. accomplishes DNA restriction
    • Discuss
    • 4. The synthesis of mRNA based on a DNA template is called

    • Options
    • A. DNA replication
    • B. transcription
    • C. translation
    • D. DNA restriction
    • Discuss
    • 5. What is the usual sequence of a Pribnow box?

    • Options
    • A. AUAUA
    • B. TATAAT
    • C. UUUUU
    • D. TTGACA
    • Discuss
    • 6. Thymine dimers are directly repaired with the help of visible light by process known as

    • Options
    • A. phosphorylation
    • B. excision repair
    • C. photosynthesis
    • D. photoreactivation
    • Discuss
    • 7. A nonsense mutation may result into

    • Options
    • A. an abnormal elongation of a polypeptide
    • B. a large deletion within the reading frame of a gene
    • C. a premature termination of the synthesis of a polypeptide
    • D. modification of mRNA
    • Discuss
    • 8. The promoter on an expression vector used to overproduce proteins in bacteria is

    • Options
    • A. a bacterial promoter
    • B. expressed both in bacteria and mammalian cells
    • C. not a regulated promoter
    • D. the natural promoter of the gene being cloned
    • Discuss
    • 9. Frame shift mutation may occur as a result of

    • Options
    • A. formation of a thymine-dimer
    • B. deamination of cytosine to uracil
    • C. conversion of guanine to xanthine
    • D. none of the above
    • Discuss
    • 10. Which of the following bacterial genome was sequenced first?

    • Options
    • A. E. coli
    • B. S. pneumoniae
    • C. H. influenzae
    • D. S. thermophilus
    • Discuss


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