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Home Microbiology Identification of Bacteria Comments

  • Question
  • What is the order of reagents used in the Gram stain?


  • Options
  • A. Crystal violet, iodine, safranin, decolorizer
  • B. Crystal violet, iodine, decolorizer, safranin
  • C. Safranin, crystal violet, decolorizer, iodine
  • D. Crystal violet, safranin, iodine, decolorizer

  • Correct Answer
  • Crystal violet, iodine, decolorizer, safranin 


  • Identification of Bacteria problems


    Search Results


    • 1. Which of the following inference(s) indicate(s) the ability of an organism to utilize citrate as a sole source of carbon in Simmon's citrate medium?

    • Options
    • A. Blue colour
    • B. Appearance of growth
    • C. Both (a) and (b)
    • D. None of these
    • Discuss
    • 2. The deinococci are gram-positive cocci and rods that are distinctive in their unusually great resistance to

    • Options
    • A. radiation
    • B. desiccation
    • C. both (a) and (b)
    • D. extreme temperatures
    • Discuss
    • 3. Which of the following is a primary stain for acid fast staining of mycobacteria?

    • Options
    • A. Crystal violet
    • B. Carbol fuchsin
    • C. Geimsa
    • D. Methylene blue
    • Discuss
    • 4. Which of the following shows a positive urease test?

    • Options
    • A. Proteus
    • B. Klebsiella
    • C. Both (a) and (b)
    • D. None of these
    • Discuss
    • 5. Which of the following(s) is/ are oxidase-positive bacteria?

    • Options
    • A. Vibrio
    • B. Neisseria
    • C. Pseudomonas
    • D. All of these
    • Discuss
    • 6. Which of the following is a substitute for crystal violet used in gram-staining procedure?

    • Options
    • A. Methylene blue
    • B. Bromocresol green
    • C. Safranin
    • D. Phenolpthalene
    • Discuss
    • 7. Production of acetoin can be detected by which of the following tests?

    • Options
    • A. Citrate test
    • B. Voges-Proskauer test
    • C. Methyl red test
    • D. Indole test
    • Discuss
    • 8. Gram staining was developed accidentally in 1884 by

    • Options
    • A. Alexander Fleming
    • B. Hans Christian Gram
    • C. Joseph Christian Gram
    • D. Robert Gram
    • Discuss
    • 9. What is the colour of gram -postive bacteria on a gram staining?

    • Options
    • A. Purple
    • B. Pink
    • C. Colourless
    • D. Green
    • Discuss
    • 10. The production of sufficient acid by fermentation of glucose leads to decrease in pH such that pH of the medium falls below 4.5. Which of the following test can detect it?

    • Options
    • A. Indole test
    • B. Methyl red test
    • C. Citrate utilization test
    • D. Voges-Proskauer test
    • Discuss


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