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  • Question
  • Which of the following methods will not sterilize?


  • Options
  • A. Aqueous glutaraldehyde for 10 hours
  • B. Dry heat at 171°C for 1 to 2 hours
  • C. 121°C at 15 lb/in pressure for 15 to 20 minutes
  • D. 100°C boiling water for 30 minutes

  • Correct Answer
  • 100°C boiling water for 30 minutes 


  • Physical and Chemical Agents problems


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    • 1. Which of the following skin disinfectant(s) is/are most frequently used?

    • Options
    • A. Isopropyl alcohol
    • B. Ethyl alcohol
    • C. Both (a) and (b)
    • D. none of these
    • Discuss
    • 2. Which of the following disinfectants act by disrupting microbial membranes?

    • Options
    • A. Cationic detergents
    • B. Halogens
    • C. Heavy metals
    • D. Aldehydes
    • Discuss
    • 3. If a canning procedure is not properly followed, which type of microbe is most likely to grow in the canned food?

    • Options
    • A. Obligate Aerobe
    • B. Acidophile
    • C. Mesophile
    • D. Obligate Anaerobe
    • Discuss
    • 4. Which of the following compounds are commonly used as general antiseptics to treat cuts and scratches?

    • Options
    • A. Aldehydes
    • B. Ethylene oxide
    • C. Halogens
    • D. None of these
    • Discuss
    • 5. Which of the following is best used for long term storage of microbial samples when carried out properly?

    • Options
    • A. Storage in a freezer at -10°C
    • B. Storage in a freezer at ultra low temperatures (-70°C)
    • C. Storage in a refrigerator on an agar slant
    • D. Storage on a petri plate at room temperature
    • Discuss
    • 6. Which of the following articles can be sterilized in an autoclave?

    • Options
    • A. Gloves
    • B. Culture media
    • C. Dressing material
    • D. All of these
    • Discuss
    • 7. Media containing spores and thermolabile constituents are sterilized by

    • Options
    • A. pasteurization
    • B. UV radiation
    • C. dry heat
    • D. tyndalization
    • Discuss
    • 8. Which of the following material is sterilized by use of red heat?

    • Options
    • A. Soiled dressings
    • B. Inoculating wires and loops
    • C. Glass slides
    • D. All-glass syringes
    • Discuss
    • 9. Which disinfectant would be most effective against Staphylococcus found in a blood spill?

    • Options
    • A. Phenol
    • B. Cetylpyridinium chloride
    • C. Hexachlorophene
    • D. None of these
    • Discuss
    • 10. Which of the following was the first widely used antiseptic and disinfectant?

    • Options
    • A. Chlorine
    • B. Phenol
    • C. Iodine
    • D. Alcohol
    • Discuss


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