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  • Question
  • Which of the following is best used for long term storage of microbial samples when carried out properly?


  • Options
  • A. Storage in a freezer at -10°C
  • B. Storage in a freezer at ultra low temperatures (-70°C)
  • C. Storage in a refrigerator on an agar slant
  • D. Storage on a petri plate at room temperature

  • Correct Answer
  • Storage in a freezer at ultra low temperatures (-70°C) 


  • Physical and Chemical Agents problems


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    • 1. Complete destruction of the microorganisms is commonly known as

    • Options
    • A. disinfection
    • B. sterilization
    • C. antisepsis
    • D. none of these
    • Discuss
    • 2. All of the following are sporicidal except

    • Options
    • A. glutaraldehyde
    • B. ethylene oxide
    • C. formaldehyde
    • D. alcohol
    • Discuss
    • 3. Iodophores are mixture of

    • Options
    • A. iodine and aldehydes
    • B. iodine and surface active agents
    • C. iodine and alcohols
    • D. iodine and phenols
    • Discuss
    • 4. Which of the following does not kill endospores?

    • Options
    • A. Autoclave
    • B. Incineration
    • C. Hot air sterilization
    • D. Pasteurization
    • Discuss
    • 5. Milk is pasteurized in batch method by keeping it at

    • Options
    • A. 63°C for 30 minutes
    • B. 72°C for 60 seconds
    • C. 73°C for 30 minutes
    • D. 72°C for 6 minutes
    • Discuss
    • 6. Which of the following compounds are commonly used as general antiseptics to treat cuts and scratches?

    • Options
    • A. Aldehydes
    • B. Ethylene oxide
    • C. Halogens
    • D. None of these
    • Discuss
    • 7. If a canning procedure is not properly followed, which type of microbe is most likely to grow in the canned food?

    • Options
    • A. Obligate Aerobe
    • B. Acidophile
    • C. Mesophile
    • D. Obligate Anaerobe
    • Discuss
    • 8. Which of the following disinfectants act by disrupting microbial membranes?

    • Options
    • A. Cationic detergents
    • B. Halogens
    • C. Heavy metals
    • D. Aldehydes
    • Discuss
    • 9. Which of the following skin disinfectant(s) is/are most frequently used?

    • Options
    • A. Isopropyl alcohol
    • B. Ethyl alcohol
    • C. Both (a) and (b)
    • D. none of these
    • Discuss
    • 10. Which of the following methods will not sterilize?

    • Options
    • A. Aqueous glutaraldehyde for 10 hours
    • B. Dry heat at 171°C for 1 to 2 hours
    • C. 121°C at 15 lb/in pressure for 15 to 20 minutes
    • D. 100°C boiling water for 30 minutes
    • Discuss


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