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Home Microbiology Microbial Recombination and Gene Transfer Comments

  • Question
  • The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is


  • Options
  • A. transformation
  • B. lysogenic conversion
  • C. conjugation
  • D. transduction

  • Correct Answer
  • transformation 


  • Microbial Recombination and Gene Transfer problems


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    • 1. The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

    • Options
    • A. have Px but not the rest of the X coding region
    • B. have the promoter of lacZ or some other reporter gene
    • C. allow to monitor the expression of all genes with a promoter similar in sequence to Px
    • D. give the same information as from a microarray
    • Discuss
    • 2. Which of the following is used by microbial genetisists as a tool?

    • Options
    • A. Bacteriophage
    • B. Plasmids
    • C. Transposable elements
    • D. All of these
    • Discuss
    • 3. Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

    • Options
    • A. Mutagenic recombimation
    • B. Site-specific recombination
    • C. Replicative recombination
    • D. General recombination
    • Discuss
    • 4. Negative regulation of protein synthesis is accomplished by

    • Options
    • A. allosteric inhibition
    • B. the binding of RNA polymerase to the promoter
    • C. the binding of a repressor to the DNA
    • D. the binding of a repressor to the RNA polymerase
    • Discuss
    • 5. Which of the following modified amino acid is used at the starting of most prokaryotic proteins?

    • Options
    • A. N-formylserine
    • B. N-formylmethionine
    • C. N-formylleucine
    • D. N-formylalanine
    • Discuss
    • 6. A microarray differs from a gene fusion in that, it

    • Options
    • A. carries DNA segments from many different genes
    • B. is not constructed by cloning
    • C. gives direct measurement of mRNA level
    • D. all of the above
    • Discuss
    • 7. Recombination of virus genomes occurs

    • Options
    • A. by transduction
    • B. by transription
    • C. simultaneous infection of a host cell by two viruses with homologous chromosomes
    • D. by transformation
    • Discuss
    • 8. The plasmids can be eliminated from a cell by the process known as

    • Options
    • A. curing
    • B. breaking
    • C. fixing
    • D. expulsion
    • Discuss
    • 9. R factors involved in plant-microbe interactions are

    • Options
    • A. plant proteins
    • B. bacterial proteins
    • C. essential for transfer of DNA to plant cells
    • D. also called opines
    • Discuss
    • 10. Which of the following statement(s) is/are true in regards to F+ x F- mating events?

    • Options
    • A. DNA is transferred from F- to F+ cells
    • B. DNA is transferred from F+ to F- cells
    • C. No DNA is transferred because F- cells are unable to perform conjugation
    • D. No DNA is transferred because F+ cells are unable to perform conjugation
    • Discuss


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