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Home Microbiology Microbial Recombination and Gene Transfer Comments

  • Question
  • The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will


  • Options
  • A. have Px but not the rest of the X coding region
  • B. have the promoter of lacZ or some other reporter gene
  • C. allow to monitor the expression of all genes with a promoter similar in sequence to Px
  • D. give the same information as from a microarray

  • Correct Answer
  • have Px but not the rest of the X coding region 


  • Microbial Recombination and Gene Transfer problems


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    • 1. Which of the following is used by microbial genetisists as a tool?

    • Options
    • A. Bacteriophage
    • B. Plasmids
    • C. Transposable elements
    • D. All of these
    • Discuss
    • 2. Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

    • Options
    • A. Mutagenic recombimation
    • B. Site-specific recombination
    • C. Replicative recombination
    • D. General recombination
    • Discuss
    • 3. Negative regulation of protein synthesis is accomplished by

    • Options
    • A. allosteric inhibition
    • B. the binding of RNA polymerase to the promoter
    • C. the binding of a repressor to the DNA
    • D. the binding of a repressor to the RNA polymerase
    • Discuss
    • 4. Which of the following modified amino acid is used at the starting of most prokaryotic proteins?

    • Options
    • A. N-formylserine
    • B. N-formylmethionine
    • C. N-formylleucine
    • D. N-formylalanine
    • Discuss
    • 5. Which of the following statement is correct?

    • Options
    • A. The size and sequence of introns can be deduced from the cDNA sequence
    • B. Restriction endonuclease can cleave ss and dsDNA both
    • C. Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a gene
    • D. Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
    • Discuss
    • 6. The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is

    • Options
    • A. transformation
    • B. lysogenic conversion
    • C. conjugation
    • D. transduction
    • Discuss
    • 7. A microarray differs from a gene fusion in that, it

    • Options
    • A. carries DNA segments from many different genes
    • B. is not constructed by cloning
    • C. gives direct measurement of mRNA level
    • D. all of the above
    • Discuss
    • 8. Recombination of virus genomes occurs

    • Options
    • A. by transduction
    • B. by transription
    • C. simultaneous infection of a host cell by two viruses with homologous chromosomes
    • D. by transformation
    • Discuss
    • 9. The plasmids can be eliminated from a cell by the process known as

    • Options
    • A. curing
    • B. breaking
    • C. fixing
    • D. expulsion
    • Discuss
    • 10. R factors involved in plant-microbe interactions are

    • Options
    • A. plant proteins
    • B. bacterial proteins
    • C. essential for transfer of DNA to plant cells
    • D. also called opines
    • Discuss


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