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Microbiology
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Microbial Recombination and Gene Transfer
Comments
Question
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will
Options
A. have Px but not the rest of the X coding region
B. have the promoter of
lacZ
or some other reporter gene
C. allow to monitor the expression of all genes with a promoter similar in sequence to Px
D. give the same information as from a microarray
Correct Answer
have Px but not the rest of the X coding region
Microbial Recombination and Gene Transfer problems
Search Results
1. Which of the following is used by microbial genetisists as a tool?
Options
A. Bacteriophage
B. Plasmids
C. Transposable elements
D. All of these
Show Answer
Scratch Pad
Discuss
Correct Answer: All of these
2. Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?
Options
A. Mutagenic recombimation
B. Site-specific recombination
C. Replicative recombination
D. General recombination
Show Answer
Scratch Pad
Discuss
Correct Answer: Site-specific recombination
3. Negative regulation of protein synthesis is accomplished by
Options
A. allosteric inhibition
B. the binding of RNA polymerase to the promoter
C. the binding of a repressor to the DNA
D. the binding of a repressor to the RNA polymerase
Show Answer
Scratch Pad
Discuss
Correct Answer: the binding of a repressor to the DNA
4. Which of the following modified amino acid is used at the starting of most prokaryotic proteins?
Options
A. N-formylserine
B. N-formylmethionine
C. N-formylleucine
D. N-formylalanine
Show Answer
Scratch Pad
Discuss
Correct Answer: N-formylmethionine
5. Which of the following statement is correct?
Options
A. The size and sequence of introns can be deduced from the cDNA sequence
B. Restriction endonuclease can cleave ss and dsDNA both
C. Restriction fragment length polymorphism (RFLP) is usually formed in coding sequence of a gene
D. Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Show Answer
Scratch Pad
Discuss
Correct Answer: Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
6. The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is
Options
A. transformation
B. lysogenic conversion
C. conjugation
D. transduction
Show Answer
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Discuss
Correct Answer: transformation
7. A microarray differs from a gene fusion in that, it
Options
A. carries DNA segments from many different genes
B. is not constructed by cloning
C. gives direct measurement of mRNA level
D. all of the above
Show Answer
Scratch Pad
Discuss
Correct Answer: all of the above
8. Recombination of virus genomes occurs
Options
A. by transduction
B. by transription
C. simultaneous infection of a host cell by two viruses with homologous chromosomes
D. by transformation
Show Answer
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Discuss
Correct Answer: simultaneous infection of a host cell by two viruses with homologous chromosomes
9. The plasmids can be eliminated from a cell by the process known as
Options
A. curing
B. breaking
C. fixing
D. expulsion
Show Answer
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Discuss
Correct Answer: curing
10. R factors involved in plant-microbe interactions are
Options
A. plant proteins
B. bacterial proteins
C. essential for transfer of DNA to plant cells
D. also called opines
Show Answer
Scratch Pad
Discuss
Correct Answer: plant proteins
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