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Home Microbiology DNA Replication Comments

  • Question
  • During DNA replication in prokaryotes, synthesis begins on the circular chromosome


  • Options
  • A. always at the same place
  • B. at any stretch of DNA that is high in AT pairs
  • C. randomly on the chromosome
  • D. at the promoter

  • Correct Answer
  • always at the same place 


  • DNA Replication problems


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    • 1. The enzyme that catalyzes the synthesis of DNA is called

    • Options
    • A. DNA polymerase
    • B. DNA gyrase
    • C. DNA ligase
    • D. helicase
    • Discuss
    • 2. During which of the following process a new copy of a DNA molecule is precisely synthesized?

    • Options
    • A. Trasformation
    • B. Transcription
    • C. Translation
    • D. Replication
    • Discuss
    • 3. The replication of chromosomes by eukaryotes occurs in a relatively short period of time because

    • Options
    • A. the eukaryotes have more amount of DNA for replication
    • B. the eukaryotic replication machinery is 1000 times faster than the prokaryotes
    • C. each chromosome contains multiple replicons
    • D. eukaryotic DNA is always single stranded
    • Discuss
    • 4. Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?

    • Options
    • A. DNA polymerases
    • B. Helicases
    • C. Single-stranded binding proteins
    • D. Topoisomerases
    • Discuss
    • 5. Proofreading activity to maintain the fidelity of DNA synthesis

    • Options
    • A. occurs after the synthesis has been completed
    • B. is a function of the 3'-5' exonuclease activity of the DNA polymerases
    • C. requires the presence of an enzyme separate from the DNA polymerases
    • D. occurs in prokaryotes but not eukaryotes
    • Discuss
    • 6. Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?

    • Options
    • A. Kornberg enzyme
    • B. DNA polymerase III
    • C. Taq DNA polymerase
    • D. None of these
    • Discuss
    • 7. DNA replication rates in prokaryotes are approximately of the order of

    • Options
    • A. 10 bases per second
    • B. 1,00 bases per second
    • C. 1,000 bases per second
    • D. 10,000 bases per second
    • Discuss
    • 8. The synthesis of DNA by DNA polymerase occurs in the

    • Options
    • A. 3' to 5' direction
    • B. 5' to 5' direction
    • C. 5' to 3' direction
    • D. 3' to 3' direction
    • Discuss
    • 9. Which of the following statements on replication in E.coli is correct?

    • Options
    • A. It occurs in a unidirectional manner
    • B. It occurs in bidirectional manner
    • C. Always uses T7 DNA polymerase when infected by T7 phage
    • D. Occurs only when ? phase has infected E. coli
    • Discuss
    • 10. DNA gyrase is inhibited by

    • Options
    • A. tetracycline
    • B. nalidixic acid
    • C. both (a) and (b)
    • D. cephalosporin
    • Discuss


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