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Home Microbiology DNA Replication Comments

  • Question
  • Which of the following enzymes unwind short stretches of DNA helix immediately ahead of a replication fork?


  • Options
  • A. DNA polymerases
  • B. Helicases
  • C. Single-stranded binding proteins
  • D. Topoisomerases

  • Correct Answer
  • Helicases 


  • DNA Replication problems


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    • 1. Proofreading activity to maintain the fidelity of DNA synthesis

    • Options
    • A. occurs after the synthesis has been completed
    • B. is a function of the 3'-5' exonuclease activity of the DNA polymerases
    • C. requires the presence of an enzyme separate from the DNA polymerases
    • D. occurs in prokaryotes but not eukaryotes
    • Discuss
    • 2. Which type of genomics studies the transcripts and proteins expressed by a genome?

    • Options
    • A. Comparative genomics
    • B. Structural genomics
    • C. Proteo genomics
    • D. Functional genomics
    • Discuss
    • 3. Which of the following theory is supported by the genomic sequence of the obligate intracellular parasite Rickettsia prowazekii?

    • Options
    • A. Parasitic bacteria have very large genomes
    • B. Parasites have a definite genomic sequence similar to viruses
    • C. Mitochondria have evolved from endosymbiotic bacteria
    • D. All bacteria evolved from viruses
    • Discuss
    • 4. What is the range of minimum set of genes required for life?

    • Options
    • A. 50-100 genes
    • B. 250-350 genes
    • C. 1000-1500 genes
    • D. 1500-2000 genes
    • Discuss
    • 5. In the time since E. coli and Salmonella diverged evolutionarily

    • Options
    • A. there has been little change in either genome
    • B. E. coli has acquired many genes via horizontal transfer
    • C. E, coli has lost approximately 50% of its genome
    • D. none of these
    • Discuss
    • 6. The replication of chromosomes by eukaryotes occurs in a relatively short period of time because

    • Options
    • A. the eukaryotes have more amount of DNA for replication
    • B. the eukaryotic replication machinery is 1000 times faster than the prokaryotes
    • C. each chromosome contains multiple replicons
    • D. eukaryotic DNA is always single stranded
    • Discuss
    • 7. During which of the following process a new copy of a DNA molecule is precisely synthesized?

    • Options
    • A. Trasformation
    • B. Transcription
    • C. Translation
    • D. Replication
    • Discuss
    • 8. The enzyme that catalyzes the synthesis of DNA is called

    • Options
    • A. DNA polymerase
    • B. DNA gyrase
    • C. DNA ligase
    • D. helicase
    • Discuss
    • 9. During DNA replication in prokaryotes, synthesis begins on the circular chromosome

    • Options
    • A. always at the same place
    • B. at any stretch of DNA that is high in AT pairs
    • C. randomly on the chromosome
    • D. at the promoter
    • Discuss
    • 10. Which of the following possesses both 5'-3' and 3'-5' exonuclease activity?

    • Options
    • A. Kornberg enzyme
    • B. DNA polymerase III
    • C. Taq DNA polymerase
    • D. None of these
    • Discuss


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