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Home Microbiology Cell Cultures and Characteristics Comments

  • Question
  • In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at


  • Options
  • A. -60 to -78°C
  • B. -20 to -30°C
  • C. -30 to -48°C
  • D. -48 to -58°C

  • Correct Answer
  • -60 to -78°C 


  • Cell Cultures and Characteristics problems


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    • 1. Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

    • Options
    • A. Pour plate
    • B. Spread plate
    • C. Both (a) and (b)
    • D. Streak plate technique
    • Discuss
    • 2. Stuart's transport medium is used for transport of specimen containing

    • Options
    • A. Neisseria gonorrhoeae
    • B. Salmonella
    • C. Vibrio cholerae
    • D. Shigella
    • Discuss
    • 3. Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

    • Options
    • A. coliform bacteria
    • B. gram positive bacteria
    • C. yeasts
    • D. acid fast organisms
    • Discuss
    • 4. Viable plate count can be obtained using

    • Options
    • A. Spread plate method
    • B. Pour plate method
    • C. both (a) and (b)
    • D. Hemocytometer
    • Discuss
    • 5. The concentration of agar to obtain semi solid media is

    • Options
    • A. 1.5-20%
    • B. 0.5% or less
    • C. >10%
    • D. >10%but<20%
    • Discuss
    • 6. In the pour plate method, the mixed culture is diluted directly in tubes of

    • Options
    • A. liquid agar medium
    • B. sterile liquid usually water
    • C. both (a) and (b)
    • D. none of these
    • Discuss
    • 7. For the selection of endospore-forming bacteria, a mixed culture can be heated at

    • Options
    • A. 80°C for 10 minutes
    • B. 70°C for 10 minutes
    • C. 60°C for 10 minutes
    • D. 90°C for 10 minutes
    • Discuss
    • 8. To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

    • Options
    • A. 8.5
    • B. 8.90
    • C. 6.79
    • D. 3.5
    • Discuss
    • 9. Important example(s) of enriched medium/media is/ are

    • Options
    • A. Loeffler's serum slope
    • B. Bordet-gengou medium
    • C. Blood agar
    • D. All of these
    • Discuss
    • 10. Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

    • Options
    • A. bile salts
    • B. low concentration of various dyes
    • C. sugars
    • D. low levels of nitrogen
    • Discuss


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