Disinfectant spectrum — All of the following agents are sporicidal (capable of killing endospores) EXCEPT:
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AGlutaraldehyde
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BEthylene oxide
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CFormaldehyde
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DAlcohol
Answer
Correct Answer: Alcohol
Explanation
Introduction / Context:Choosing the right chemical for high-level disinfection or sterilization requires awareness of sporicidal activity. Endospores resist many routine disinfectants; only a subset of chemicals are validated as sporicidal under correct conditions (concentration, temperature, exposure time).
Given Data / Assumptions:
- We compare high-level agents (aldehydes, ethylene oxide) with intermediate agents (alcohols).
- “Sporicidal” implies proven kill of bacterial spores in standardized tests.
Concept / Approach:Glutaraldehyde and formaldehyde (aldehydes) crosslink proteins and nucleic acids, achieving sporicidal effects with sufficient exposure. Ethylene oxide gas alkylates cellular constituents, sterilizing heat-sensitive equipment (including spores). Alcohols (ethyl, isopropyl) are excellent skin antiseptics but are not reliably sporicidal.
Step-by-Step Solution:
Identify aldehydes (glutaraldehyde, formaldehyde) as potential sterilants.Note ethylene oxide as a gaseous sterilant used for devices and catheters.Recognize alcohols as non-sporicidal; select as the exception.Verification / Alternative check:Disinfection guidelines categorize alcohols as intermediate-level (bactericidal, tuberculocidal, virucidal) but not sporicidal; aldehydes and ethylene oxide meet sporicidal criteria with validated protocols.
Why Other Options Are Wrong:
- Glutaraldehyde: sporicidal at recommended exposure (e.g., ≥2% solutions).
- Ethylene oxide: sterilant gas for heat-sensitive instruments.
- Formaldehyde: sporicidal with adequate concentration/time.
Common Pitfalls:Assuming strong “kill” on vegetative cells equals spore kill; spores need higher-level chemistry or heat.
Final Answer:Alcohol