Autoclave suitability: Which articles listed are appropriate for sterilization by steam under pressure (autoclaving) using standard wrapped-cycle conditions?

Difficulty: Easy

Correct Answer: All of these

Explanation:


Introduction:
Autoclaving is the workhorse sterilization method in microbiology and surgery. Items that tolerate moisture and heat can be reliably sterilized by saturated steam at 121–134°C.


Given Data / Assumptions:

  • Aqueous culture media are formulated for autoclaving.
  • Dressing materials and surgical packs are routinely steam-sterilized.
  • Reusable rubber items (e.g., surgical gloves/tubing) can be autoclaved if manufacturer allows.


Concept / Approach:
Steam transfers heat efficiently and penetrates porous loads. Autoclaving denatures proteins and nucleic acids, destroying spores when time/temperature are sufficient. The common denominator is moisture and heat stability.


Step-by-Step Solution:
Check each item for heat/moisture compatibility. Media → yes; dressings → yes; reusable rubber gloves → yes (per IFU). Therefore, choose the inclusive option.


Verification / Alternative check:
Hospital CSSD workflows and lab SOPs list these categories for wrapped steam cycles with biologic/chemical indicators to verify sterility assurance level.


Why Other Options Are Wrong:
Single selections are incomplete; “None” contradicts standard practice.


Common Pitfalls:
Confusing disposable exam gloves (not autoclaved) with reusable sterile gloves supplied after autoclaving in packs; the question refers to items suitable in principle.


Final Answer:
All of these.

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