Outcome of transcription—what molecule is produced? During gene expression, transcription directly produces which type of nucleic acid product?

Introduction / Context:
Transcription is the first major step of gene expression, converting information encoded in DNA into an RNA message that can be translated into protein or used as a functional RNA.

Given Data / Assumptions:

• DNA serves as a template.
• RNA polymerase catalyzes ribonucleotide addition.
• Product depends on gene class: mRNA, rRNA, tRNA, etc.

Concept / Approach:
When referring to “gene expression” in protein-coding genes, the immediate product of transcription is messenger RNA (mRNA). Although other RNAs are also made by transcription, the standard answer emphasizes mRNA as the template for translation into a polypeptide.

Step-by-Step Solution:
Identify process: transcription (DNA → RNA).Identify product for protein-coding genes: mRNA.Select mRNA from the options provided.

Verification / Alternative check:
In vitro transcription reactions with DNA templates yield RNA transcripts detectable by RNA-specific labeling or RNase sensitivity.

Why Other Options Are Wrong:

• Amino acid chain: Product of translation, not transcription.
• cDNA: DNA made from RNA by reverse transcriptase; not a direct transcription product.
• Okazaki fragments: DNA replication intermediates.
• Ribosomal subunits: RNP assemblies; rRNA is transcribed, but subunits are not the direct product.

Common Pitfalls:
Conflating transcription with translation or replication intermediates.

Final Answer:
Messenger RNA (mRNA).