AT-rich promoter motifs across domains In both prokaryotes and eukaryotes, the classic AT-rich promoter element is commonly known as the:
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ACATT box
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BShine–Dalgarno sequence
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CTATA box
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DSV40 origin region
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EGC box
Answer
Correct Answer: TATA box
Explanation
Introduction / Context:Promoters contain characteristic sequence motifs that recruit transcription machinery. One of the best-known is the AT-rich TATA element, found in many genes (though not all) across domains.
Given Data / Assumptions:
- TATA elements are AT-rich and facilitate DNA unwinding.
- Eukaryotes use TATA-binding protein (TBP) as part of TFIID; bacteria position the -10 element (Pribnow box) which is also AT-rich.
Concept / Approach:The “TATA box” refers to an AT-rich promoter element that helps position RNA polymerase and initiate transcription. While exact sequences differ (eukaryotic TATA box, bacterial -10 consensus), the testable name for an AT-rich promoter motif is TATA box.
Step-by-Step Solution:Identify the AT-rich element: TATA-like motif.Exclude unrelated sequences (Shine–Dalgarno is a ribosome-binding site in prokaryotic mRNAs, not a promoter box).Select “TATA box.”
Verification / Alternative check:Promoter mutagenesis shows loss of transcription when TATA is disrupted; TBP footprinting confirms recognition.
Why Other Options Are Wrong:
- Shine–Dalgarno: Translation initiation, not transcription initiation.
- SV40 origin / GC or CATT boxes: Distinct regulatory/replicative elements; not the canonical AT-rich promoter box referred to here.
Common Pitfalls:Mixing up translation and transcription signals.
Final Answer:TATA box.