Difficulty: Easy
Correct Answer: Promoters
Explanation:
Introduction / Context:
Accurate transcription requires specific DNA sequences that recruit RNA polymerase and associated factors. In both prokaryotes and eukaryotes, these core elements define the transcription start site and basal transcription levels.
Given Data / Assumptions:
Concept / Approach:
Promoters contain consensus motifs (e.g., −35/−10 in bacteria; TATA box in many eukaryotic genes) that determine polymerase binding and transcription initiation. Enhancers are regulatory DNA segments that can act at a distance but are not the core start site determinants.
Step-by-Step Solution:
Match definition: essential, upstream, and required for initiation → promoter.
Differentiate from start codons (translation, not transcription).
Separate from enhancers/silencers (modulate level and timing but are not the core start elements).
Verification / Alternative check:
Mutations in promoter motifs reduce or abolish transcription; swapping promoters redirects expression.
Why Other Options Are Wrong:
Transcription factors are proteins, not DNA regions; start codons function in translation; enhancers/silencers are distal regulatory elements.
Common Pitfalls:
Confusing promoter (DNA) with polymerase (protein); conflating transcription and translation terminology.
Final Answer:
Promoters.
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